Clinical characteristics and molecular characteristics of\r\n', u'hepatitis B virus in chronic hepatitis B virus infected patients\r\n', u'with A1899 mutation in precore region\r\n', u'

Background: Hepatitis B is an infectious illness caused by hepatitis B virus (HBV) which infects the liver of hominoidea, including humans, and causes an inflammation called hepatitis. Objectives: The aim of study is to clarify clinical features and molecular characteristics of HBV in chronic HBV-infected patients with A 1899 mutation. Subjects and method: HBV genotype, HBV-ONA level, HBeAg and anti-HBe in 29 chronic HBV-infected patients were determined by PCR-RFLP, Real-time PCR and ELISA, respectively. Mutations were analyzed by direct sequencing. Results: Mutations in core-promoter/precore regions of HBV genome can suppress HBeAg secretion and stimulate HBV-ONA replication. The prevalence of hepatocel- lular carcinoma (HCc): 10/29, liver cirrhosis (LC) : 15/29 are significantly higher than that in chronic hepatitis (CH) : 4/29 (P < 0.001). HbeAg seroconversion rate in CH (75%) is higher than that in HCC \r\n', u'(40%) and in LC (53.3%), but not significant (P > 0.05). ALT level is the highest in CH and the lowest in HCC \r\n', u'(P = 0.02), 8/10 (80%) HCC patients have normal range of ALT. HBV-ONA level in HCC and in LC is significantly higher than that in CH (P = 0.024). The emerging of A 1899 is often accompanied by C/G1753 mutation (37.9%) and dual core-promoter mutation T1762A1764 (79.3%). Conclusion: A1899 mutation can play a role in the pathogenesis of liver diseases in chronic HBV-infected Vietnamese.\r\n', u'

The change of cytological components of platelet mass prepared and stored in different conditions

Background: Random donor platelet concentrations (PCs) can be prepared by 2 methods: from platelet rich plasma (PRP) and from buffy coat (BC). In this study, the changes of cytological properties of the PCs prepared and stored at the different conditions were evaluated. Method: 45 samples of whole blood were divided into 3 groups: PCs-PRP stored at 22oC with shaking (n=15), PCs-BC stored at 22oC with shaking and PCs-PRP stored at 4oC without shaking. The assessment of platelet, red cell, white cell counts and hypotonic shock responses (HCR) were performed. Result: the yield of platelet separation of PRP method is higher and the leukocyte contamination is lower than those of BC method are. During storage, the platelet loss is highest in PCs-PRP stored at 4oC in comparison with PCs stored at 22oC. The HSR of PCs-PRP stored at 22oC reached the best results at 1st day and was stable up to 5th day. Conclusion: The PCs-PRP stored at 22oC showed the advantages in platelet yield, the leukocyte contamination and, also, the HSR

Comparison of the yield of platelet separation between two methods: platelet rich plasma (PRP) and buffy coat (BC)

Production of platelet concentrates (PCs) have an important role in the replacement therapy for severe thrombocytopenic conditions. PCs can be prepared by centrifugal technique which have two main methods: platelet rich plasma and buffy coat. We studied on 40 blood units (volume 450 ml, anticoagulant: CPD-A1, triple bags-type) and evaluated the yield of platelet (PLT) separation red blood cell (RBC) and white blood cell (WBC) contamination from these methods. The result show that the yield of PLT separation from PRP method is significantly higher than those from BC (82.60+ 7.20x1010, yield: 56.75% vs 50.59 + 2.97 yield: 32.4%) The WBC and RBC’s contamination in PCs from two methods are higher than the standard of Council of Europe. By sum up, follow studies will be necessary for decreasing the WBC and RPC’s contamination in PCs, the PRP method may be evaluated as a high-yield method in PCs production

Changes of platelet count, white blood cell count, glucose concentration and pH in platelet concentrates during the four day storage

We studied in 40 platelet concentrates (PCs) prepared by two methods: Platelet-rich plasma (PRP) (n= 20) and buffy coat (BC) (n=10) and evaluated the changes of platelet count, white blood cell count, glucose concentration and pH during the 4 days-storage. There was not significant difference between two groups for platelet count, glucose concentration at the day 0. After 4 days, the platelet’s loss in PCs- PRP was significantly lower than that in PCs-BC (4.93x 109 Vs 7.14 x 109, p<0,05) and PCs- PRP had the lower glucose consumption than PCs- BC (53.6 mg% vs 115.9 mg%, non significant). The WBC counts and pH were higher in PCs- PRP than those in PCs-BC at day 0, but the WBC’s decreasing during the storage as not different between two groups, the pH of PCs-BC reduced more quickly than that of PCs- PRP are more stable than those in PCs- BC during 4 day storage.