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OBJECTIVE To evaluate the detect method of neopterin(Npt) by enzyme-linked immunosorbent assay(ELISA) and discuss the application of Npt in the viral encephalitis.METHODS The Npt was detected by ELISA.The methodology was investigated by sensitivity,precision,rate of recovery,interference and reference value.The npt was detected by ELISA assay in peripheral blood of 50 healthy people,30 cases of cerebrospinal fluid in children with viral encephalitis and 12 cases of cerebrospinal fluid in the control group.RESULTS In this method,within-run CVs were 4.94% and 5.55%;between-run CVs were 5.99% and 6.57%.The sensitivity was 1.08 nmol/L;the rate of recovery was 95.8-107.8%.Various indexes of the methodology coincided with the requirements of clinical laboratory.The reference value of serum Npt was 0-7.84 nmol/L.The Npt of cerebrospinal fluid was(34.09 ? 36.34) nmol/L in the viral encephalitis group,and(4.55 ? 2.89)nmol/L in the control group,and the Npt of cerebrospinal fluid in patient was significantly higher than that in control group(P
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OBJECTIVE To analyze the pathogenic results and drug resistance of bacteria isolated from serous effusion specimens from Jan 2006 to Feb 2008 so as to provide evidence for clinical pathogenic analysis and selection of antibiotic. METHODS The bacteria identification and the antimicrobial susceptibility were assayed by routine methods. RESULTS The positive rate of pathogens was 6.80% from 721 serous effusion specimens.There were 49 strains of pathogens which included 15 of Gram-positive bacteria and 28 of Gram-negative bacteria and 6 fungi strains.The most frequently isolated pathogens were Enterobacteriaceae(32.65%) followed by Enterococcus(18.37%),and Pseudomonas aeruginosa(12.25%).The most active compounds against Enterococcus were vanconmycin and linezolid(100.00%),nitrofurantoin and tetracycline(66.67%);the most active compounds against Staphylococcus were vancomycin,linezolid and nitrofurantoin(100.00%),tetracycline and rifampin(75.00%);The most active compounds against Gram-negative bacilli were piperacillin/tazobactam(67.86%),imipenem(64.29%),tobramycin(60.71%),gentamicin(53.57%) and levofloxacin(46.43%). CONCLUSIONS The prevailing pathogens in serous effusion are Gram-negative bacilli,especially Enterobacteriaceae.Data collected in present study provide a valuable information for prophylactic and empirical antibiotic use for serous infection.
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OBJECTIVE To analyze the pathogenic results and drug resistance of bacteria isolated from cerebrospinal fluid specimens from Jan 2006 to Nov 2007 so as to provide evidence for clinical pathogenic analysis and selection of antibiotic.METHODS The bacteria identification and the antimicrobial susceptibility were assayed by VITEK-AMS system.RESULTS The positive rate of pathogens was 11.62% from 878 cerebrospinal fluid specimens.There were 102 strains of pathogens which included 19 Gram-positives,79 Gram-negatives and 4 fungi.The most frequently isolated pathogen was Pseudomonas(41.18%)followed by Staphylococcus haemolyticus(9.80%),Escherichia coli(8.82%),Acinetobacter spp(5.88%),Staphylococcus epidermidis(4.90%)and Shewanella putrefaciens(4.90%).The most active compounds against Gram-positive cocci were vancomycin(100.00%),linezolid(94.74%),nitrofurantoin(84.21%),tetracycline(78.95%),and rifampin(73.68%);The most active compounds against Gram-negative bacilli were piperacillin/tazobactam(75.95%),cefepime(58.23%),imipenem(54.43%),ciprofloxacin(53.16%) and levofloxacin(48.10%).CONCLUSIONS The prevailing pathogens in cerebrospinal fluid are Gram-negative bacilli,especially Pseudomonas and E.coli.Data collected in present study provide valuable information for prophylactic and empirical antibiotic use in intracranial infection.