ABSTRACT
Objective To investigate protective effects and mechanisms of lipoxinA4(LXA4)on human umbilical vein endothelial cells(HUVEC)under hypoxia in vitro.Methods The HUVEC culture were divided into groups as followed:added M199 cudture medium as normal contml groups,added CoCl2 to mimic hypoxia in vitro as hypoxia group and added different concentrations of LXA4(1,10,100 nmol/L)were added to the induced hypoxiai HUVEC as agents intervention group.Morphological changes of HUVEC were observed by using inverted phase contrast mieroscope.The influence of LXA4 on cell survival was investigated by methyl thiazolyl tetrazolium(MTT) assaying method after the treatment with different concentrations of LXA4 and 100 nmol/L lipoxinA4 according to different time(4,8,12 and 24 hours).The expression of von-willebrand factor (vWF) was detected by immunocytoehemistry method. The changes of cytosolic Ca2+ were measured by laser scanning confocal microscope. Results ( 1 ) Morphological changes:the cells under hypoxia lost its normal shapes and showed necrosis, while the cells cocuhured with 100 nmol/L LXA4 were normal appropriately. (2)Survival rate: the survival rates of HUVEC under hypoxia was (40. 1±3.9) % and increased to ( 52. 9 ± 1.4) %, (64. 1 ± 3. 3 ) %, ( 76. 6 ± 1.6) % respectively when added with LXA4 with concentration of 1,10, 100 nmol/L into culture medium. There was significant different survival rate when compared with that of hypoxia group. (3) The level of vWF: The expression of vWF was decreased with the increasing concentrations of LXA4 added into culture medium, the gray values were 203.9 ±0. 7 in 1 nmol/L,204.6 ±0. 9 in 10 nmoL/L,191.8 ±0. 5 in 100 nmol/L respectively, which reached statistical difference in comparison with that of hypoxia groups (P<0. 05). (4) Confocai analysis:the intracellular free Ca2+ concentrations of HUVEC were intensified with LXA4 treatment. Conclusions LXA4 plays an important role in keeping the normal shape of HUVEC under hypoxia, can enhance survival of hypoxial HUVEC and decrease the level of vWF in cytoplasm. The protective mechanism might be via decreasing mitochondria Ca2+ overload and increasing cytoplasm Ca3+ by nucleus Ca2+ transference.
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Objective To test the timeliness of newly developed electronic medical tag system.Methods According to a standardized logistical process of medical tag in battlefield,timeliness tests of electronic medical tag and paper-based medical tag in two different echelons: battalion-company and medical battalion were completed,and the data of two groups were compared.Results It showed that the consumed time in the electronic medical tag system was 3/10 and 1/11 of the consumed time in paper-based medical tag respectively.Conclusion The timeliness of the electronic medical tag system is much better than that of the paper-based medical tag and meets the timeliness requirements of treatment in battlefield.
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Objective To select a electronic medical tag system suitable for modern war. Methods The active radio frequency identification technology was surveyed, modeled, and improved. Results It was demonstrated be tests that active radio frequency identification technology met the requirements of electronic medical tag system. Conclusion The electronic medical tag system based on radio frequency identification technology changes the traditional working mode and enhances integral capability of field first aid.
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@#用秩和比法对37例脑瘫患者施行SPR术中影响应激反应的诸因素进行分析。结果显示：3—6岁小儿、切断后根小束大于50％者、电刺激量平均为5．1—10mA者、芬太尼量大于3μg／kg·h者和于腰骶部行SPR者应激反应最强。3、4级痉挛者、芬太尼量为2．1—3μg／kg·h者、7—13岁儿童、切断后根小束41—50％者和电刺激量平均大于10mA者应激反应较强。5级痉挛、14岁以上青成年和电刺激量平均小于5mA者，应激反应较弱。而芬太尼量小于2μg／kg·h、切断后根小束少于40％和行颈胸段SPR者应激反应最弱。经临床分析，作者提出：SPR中应特别注意6岁前小儿及切断后根小束超过50％的脑瘫患者的应激反应。
ABSTRACT
@#cases were classified by using RUGⅢ.The result indicates that RUG Ⅲ is objective to re-flect all reasonable costs for clients.It has therotical and practical significence to research RUG Ⅲ for institu-tions involving rehabilitation,family beds and deathbed care.
ABSTRACT
Objective To develop electronic medical tag system on scientifically,rationally,orderly and efficiently.Methods By analyzing total goals of system development and application requirement of military medical logistics to design rational-ly system function,scan carefully and examine application technology,insist on the independent development and innova-tion,focus on the standardization of equipment and information.Results Through the various tests of the system,the per-formance of the whole system can be met the requirements of tactical specification and perfect effect.Conclusion The de-velopment of equipment must be grasped the military requirements and the technical and non-technical component ele-ment,focus on the technical breakthrough and follow standardization requirements,then the quality of equipment can be guaranteed,and the task of serving for medical support can be accomplished.