In vitro lipid peroxidation of tissue cultured and tissue culture derived mentha plant.

The effect of tissue cultured and tissue culture derived Mentha species viz. Mentha piperita(PPR611), Mentha arvensis (SH) and Mentha spicata (SPR 8) on antioxidant potential using lipid peroxidation model was studied. The extracts prepared were analyzed for total phenols and flavonoids. Tissue cultured derived plants were found to have higher content of total phenols and flavonoids. Furthermore, Mentha spicata (SPR 8) was found to possess higher content of total phenols, however, flavonoid content of tissue cultured samples was more compared to tissue culture derived plants in Mentha arvensis (SH) and Mentha spicata (SPR 8).Tissue cultured plants of all the three species were more potent in lipid peroxidation inhibition model. Tissue cultured derived plants were less effective in preventing lipid peroxidation and inhibition potential decreased with development period in all the three species.

In vivo antioxidant potential of rice bran oil (RBO) in albino rats.

Rice Bran Oil (RBO) has got many health benefits. RBO has been analyzed for physico-chemical characteristics and compared with those of groundnut oil (GNO). The two oils were similar in various physicochemical characteristics. The major difference in the two oils lay in the amount of unsaponifiable matter, which was higher in the case of RBO. To find the in vivo antioxygenic potential of RBO, particularly its ability to protect against oxidative stress, rats were divided into two groups of 10 animals, each and were maintained on diets containing RBO or GNO for a period of 4 weeks. After which stress was induced to half the animals of each group by administering intraperitoneally N-nitrosodiethylamine (NDEA) (100 mg/kg) body weight and remaining half served as respective controls. Animals were sacrified 1 week after stress induction. Intraperitoneal administration of NDEA resulted in a significant reduction in body weight and feed intake, the effect being appreciably less in RBO fed group. NDEA toxicity was mainly reflected in liver as supported by increased activities of enzymes of liver function test (AST, ALT, ALP) on stress induction but the effect was appreciably of lesser degree in the group fed on RBO. The urea levels were also less in the group fed on RBO, The lipid peroxidation (LPO) increased on stress induction in erythrocytes and in all the tissues, the increase being less in RBO fed group except in kidneys. Stress induction resulted in decreased catalase (CAT) activity, the decrease being less in RBO fed group. The increase in peroxidase (Px) activity on stress induction was more in RBO fed group. Stress induction had no significant effect on superoxide-dismutase (SOD) activity except in liver and heart where it increased on stress induction. Thus, it appears that inclusion of RBO in the diet improves the antioxygenic potential and protect against oxidative stress.

Impact of hypercholesterolemia on in vitro toxicity of N-nitrosodiethylamine: effect on lipidperoxidation of blood and tissue.

In vitro treatment of erythrocytes of normal and hypercholesterolemic rats with N-nitrosodiethylamine (NDEA), an important carcinogen frequently present in human environment and food chain resulted in a marginal increase in osmotic fragility of erythrocytes without affecting their antioxygenic potential as evidenced by insignificant effect on lipid peroxidation (LPO). However, (LPO) of all the tissues (heart, lung, liver, kidney and spleen) increased significantly on in vitro treatment with NDEA. The effects were different in different tissues under different dietary conditions