ABSTRACT
OBJECTIVE: To examine the effect of resveratrol on cell proliferation and cell cycle progression in the human uterine leiomyoma cells. METHODS: MTS [3-(4,5-dimethylthiazol-2-yl)5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] reduction assay was carried out to determine the viability of human uterine leiomyoma cells. Western blot analysis was done using anti pRB, anti-p21cip1/waf1, anti-p53, anti-cyclin E, anti CDK2 antibodies to detect the presence and expression of these proteins in treatment with resveratrol. DNA fragmentation assay was done to find the rate of apoptosis. Cell cycle analysis for resveratrol treated in human uterine leiomyoma cells was done by FACS (fluorescence-activated cell sorter) analysis. RESULTS: Resveratrol induced growth inhibition in a dose dependent manner, treatment with 100 ?M/L resveratrol blocked 30% cell growth. From Western blot analysis it revealed resveratrol induced the expression of p53 increasing. Caspase pathway was activated and cleavage of PARP was occurred. Apoptosis took place but in a reduced manner. FACS results showed that resveratrol increased the percentage of cells in sub G1 phase. CONCLUSION: Resveratrol, a dietry phytoalexin, inhibited cell proliferation and induced cell cycle arrest at sub G1 by enhancing the production of p53. These results indicate that resveratrol will be a promising agent chemopreventives or therapeutics against human uterine leiomyoma cells.
Subject(s)
Humans , Antibodies , Apoptosis , Blotting, Western , Cell Cycle , Cell Cycle Checkpoints , Cell Proliferation , DNA Fragmentation , G1 Phase , LeiomyomaABSTRACT
This paper reflects on the standardization activities of nursing documentation. Even though nurses are the most important manpower in terms of collecting patients' data, nursing documentation have been overlooked in the process of developing electronic patients records. It is impossible to complete a computerized patient record system without including nursing documentation. Standardization of nursing documentation is the first step toward a computerized documentation system. In this study nursing documentation forms were gathered from 11 tertiary hospital with more than 500 beds in Seoul. Out of various nursing documentation, 9 essential forms were chosen to standardize. They are admission assessment, form, nursing treatment record, nursing care plan, discharge planning record, patient transfer record, clinical observation record, nursing treatment record, nursing progress notes, critical care flow sheet, and preoperative checklist Forms and data elements were reviewed and analyzed. It was learned that there is no one perfect from that could be used in any agency. Data elements were analyzed and standardized. Data elements to be included in each form were selected. Standardized forms were developed with the selected data element. Guideline outlining how to use each nursing form were developed. Now it is in the process of validating the forms and the guidelines at 240 nursing units at 8 tertiary hospitals. The results of the validation study will be incorporated in the final version of nursing forms and they will be introduced to general nursing population at an open forum to be held by Korean Nurses Association at the end of this year. This standardization activities will have a great impact on nursing practice, education, administration and research.
Subject(s)
Humans , Checklist , Critical Care , Education , Health Records, Personal , Nursing Informatics , Nursing Records , Nursing , Patient Discharge , Patient Transfer , Seoul , Tertiary Care CentersABSTRACT
Male breast cancer is very rare, with the incidence of 0.15-1% of all breast cancers and less than 1% of all cancers in men. The prognosis of male breast cancer is poorer than that of female because the median age of detection of the disease is in more late stage. It usually involves higher axillary lymph nodes. We report two cases of male breast cancer to describe characteristic mammographic and ultrasonographic findings and to compare with the findings of gynecomastia.
Subject(s)
Female , Humans , Male , Male , Breast , Breast Neoplasms, Male , Gynecomastia , Incidence , Lymph Nodes , PrognosisABSTRACT
Alopecia has been described as associated with diseases of the endorine glands, various tension state and emotional shock, and as a result of reflex irritation from traumatic injuries. Recently we have observed 3 cases of distinctive type of alopecia after long timed microvascular surgery in which a long-standing pressure to the occiput, seemed to be responsible. All patients noted swelling, exudation and crust formation over the occiput shortly after the operation and usually within the 2 days. Some times there was tenderness. Hair loss occurred from 7 days and regrowth of hair appeared from 30 days with non-specific therapy to hair loss. Histopathologic microscopic findings showed that there were mild chronic perivascular inflammatory reactions.
Subject(s)
Humans , Alopecia , Hair , Reflex , ShockABSTRACT
It may be the most important problem to keep the patent airway during a general anesthetic procedure or when emergency patient care is necessary. Endotracheal intubation facilitates to keep the airway. But unfortunately, we are confronted with complications of endotracheal intubation, occasionally. The authors experienced a case of segmental bronchial obstruction with mucus plug and successfully remedied by fiberoptic bronchoscopic aspiration through the endotracheal tube.