ABSTRACT
Granulicatella adiacens was first described as nutritionally variant streptococci, and named as Streptococcus adjacens and Abiotrophia adiacens. Granulicatella species are flora of oral cavity, upper respiratory, urogenital, and gastrointestinal tracts, and are normal often isolated from patients with infective endocarditis. G. adiacens endocarditis is associated with high mortality, but the species hardly grow in the ordinary growth media. We report a case of septicemia caused by G. adiacens in a 52-year-old woman with liver cirrhosis and chronic renal failure.
Subject(s)
Female , Humans , Middle Aged , Abiotrophia , Endocarditis , Gastrointestinal Tract , Kidney Failure, Chronic , Liver Cirrhosis , Mortality , Mouth , Sepsis , StreptococcusABSTRACT
Granulicatella adiacens was first described as nutritionally variant streptococci, and named as Streptococcus adjacens and Abiotrophia adiacens. Granulicatella species are flora of oral cavity, upper respiratory, urogenital, and gastrointestinal tracts, and are normal often isolated from patients with infective endocarditis. G. adiacens endocarditis is associated with high mortality, but the species hardly grow in the ordinary growth media. We report a case of septicemia caused by G. adiacens in a 52-year-old woman with liver cirrhosis and chronic renal failure.
Subject(s)
Female , Humans , Middle Aged , Abiotrophia , Endocarditis , Gastrointestinal Tract , Kidney Failure, Chronic , Liver Cirrhosis , Mortality , Mouth , Sepsis , StreptococcusABSTRACT
BACKGROUND: The test for hippurate hydrolysis is critical for differentiation of C. jejuni and other thermophilic Campylobacter species. So, we evaluated the disk method for detection of hippurate hydrolysis by C. jejuni. METHODS: Twenty-eight Campylobacter species isolated from stool culture were simultaneously tested with disk method for detection of hippurate hydrolysis and polymerase chain reaction (PCR) for hippuricase specific gene. Disk method was tested with difference in incubation time (2 hours vs. 4 hours), hippurate concentration (1%, 2%, and 4%), amount of ninhydrin (50 microliter vs. 100 microliter), and inoculation method (colony vs. suspension of organism adjusted by turbidity), finally, 24 types of disk methods were performed. RESULTS: By using hippuricase PCR method as the reference for the detection of hippurate hydrolysis, the disk method showed a sensitivity of 91.7% and a specificity of 100% when two kinds of disk methods were simultaneously performed. CONCLUSIONS: The disk method for detection of hippurate hydrolysis is simple to use and require fewer cells than the tube method do, and should be useful as a routine diagnostic test in clinical laboratory for rapid identification of C. jejuni.
Subject(s)
Campylobacter jejuni , Campylobacter , Diagnostic Tests, Routine , Hydrolysis , Ninhydrin , Polymerase Chain Reaction , Sensitivity and SpecificityABSTRACT
BACKGROUND: Vancomycin-resistant enterococci (VRE) were first recovered from clinical isolates in Korea in 1992, and the incidence has been steadily increasing. Alternatives to vancomycin are few because VRE are frequently resistant to commonly used antimicrobial agents. The present study was designed to assess the in-vitro activity of fosfomycin to clinical isolates of VRE. METHODS: For 199 VRE isolates from 1995 to 2000, and 91 enterococcal isolates that were consecutively isolated during the January of 2001 at Wonju Christian Hospital, fosfomycin (200 microgram) disk diffusion test was done by NCCLS method. The number of enterococcal isolates tested for fosfomycin were as follows:58 E. faecalis (42 vancomycin susceptible isolates, 16 vancomycin resistant isolates, and 1 vancomycin intermediate resistance isolate); 210 E. faecium (185 vancomycin resistant and 25 vancomycin susceptible isolates); 15 E. gallinarum, and 6 E. casseliflavus isolates. RESULTS: Among the VRE isolates, the resistance rates of fosfomycin according to enterococcal species were 6.3% in E. faecalis, 4.9% in E. faecium, 0% in E. casseliflavus, and 16.7% in E. gallinarum. CONCLUSION: Fosfomycin could be a potentially useful drug for the treatment of infections caused by VRE.