ABSTRACT
Epulis Fissuratum [Epulis Fissuratum [EF] or Denture Epulis or inflammatory fibrous hyperplasia] is a common hyperplastic tumor-like lesion with reactive nature, related to loose and ill-fitting, full or partial removable dentures and it is more common in women than men. For this reason, hormonal influences may also play role in its creation. The effect of steroid hormones especially sex hormones [Estrogen and progesterone] on oral mucosa is identified in some studies. In the present study, the distribution pattern and presence of estrogen and progesterone receptors in epithelial, stromal, endothelial and inflammatory cells in Epulis Fissuratum was investigated. This cross-sectional study was carried out on 30 samples of paraffin blocks with Epulis Fissuratum diagnosis and 30 samples of normal mucosal tissues as a control group who have had surgery as a margin beside the above lesions and had been obtained from the oral and maxillofacial pathology departement of Babol Dental School since 2003 up to 2010. Intensity of staining and immunoreactivity were evaluated using subjective index and considering the positive control group [breast carcinoma]. Epithelial, stromal, endothelial and inflammatory cells didn't show reaction with monoclonal antibodies against estrogen and progesterone in none of the samples. It seems that the hypothesis of the existence of estrogen and progesterone receptors in epulis fissuratum and normal oral mucosa is ruled out. The possibility of direct effect of estrogen and progesterone in occurring of epulis fissuratum is rejected
Subject(s)
Humans , Male , Female , Receptors, Progesterone , Receptors, Estrogen , Immunohistochemistry , Cross-Sectional StudiesABSTRACT
Ameloblastoma is a benign epithelial odontogenic tumor that exhibits a more aggressive behavior than odontogenic keratocyst and follicular cyst. The purpose of this study was to determine and evaluate histomorphometric characteristics and blood vessels pattern adjacent to and far from odontogenic epithelium on the clinical behavior of the above odontogenic lesions with immunohistochemistry and morphometry method. In this descriptive-cross-sectional study, 45 paraffin blocks of mentioned lesions were selected and marked immunohistochemically with CD34. From three HPFs [High Power Fields] in each slide which had more density of blood vessels in adjacent to and far from odontogenic epithelium, photography was done by a camera joined to a microscope with 40 times magnification. Through the software Motic plus 2, the internal and external diameters, section surface and thickness were determined. Also the blood vessel pattern was determined in the mentioned lesions and was compared through ANOVA, f-test and Multiple Comparison Test. The external and internal diameters and sectional surface in the regions far from the odontogenic ephithelium in ameloblastoma was greater than keratocyst [P<0.001] and dentigerous cysts [P=0.001]] but there was no significant statistical differences seen in the thickness of blood vessels in the regions far from the odontogenic ephithelium in three lesions [P=0.05]. Significant statistical difference was not seen in external [P=0.8] and internal [P=0.8] diameters, sectional [P=0.6] surface and blood vessels thickness in the adjacent regions of odontogeni ephithelium in these three lesions. The pattern of blood vessels in ameloblastoma was circumferential and in the keratocyst and follicular cyst, it was directional. It seems that morphometric characteristics of blood vessels [internal and external diameters, sectional surface] in areas far from odontogenic ephithelium and the type of blood vessels pattern affects clinical aggressive behavior of ameloblastoma more than keratocyst and follicular cyst
ABSTRACT
The aim of this prospective study is to determine the relationship between the pathologic characteristics of the transitional cell carcinoma [TCC] of the bladder and prostatic involvement. Sixty men with bladder TCC underwent standard radical cystoprostatectomy and were enrolled in this study. Vascular and perineural invasion, maximum diameter of the tumor, presence of carcinoma in situ, distance between the tumor and the bladder neck, and grade and local stage of the tumor were recorded and their relation with prostatic involvement was studied. In addition, hydronephrosis and age of the patients were included in the analysis. The mean age of the patients was 63.9 +/- 11.1 years. Of 60 men included in this study, 15 patients were found to have prostatic involvement with TCC [25%]. Univariate statistical analyses showed that vascular invasion and the distance between the tumor and the bladder neck were significantly related to the prostatic involvement [P = .007; P < .001]. But, in the logistic regression, only the distance between the tumor and the bladder neck was significantly related to the prostatic involvement [P = .001]. This study suggests that the probability of prostatic involvement in patients with bladder TCC tumors near the bladder neck is high. Prostate-sparing or capsule-sparing cystectomy should be avoided in such patients