ABSTRACT
Ocotea duckei , known as Louro - de - cheiro, belongs to the Lauraceae family and presents lignoid yangambine (YAN) as the main plant marker. This work aimed to develop and validate an analytical method by high performance liquid chromatography for the quantification of YAN. The sample used was the crude eth anolic extract (CEE) obtained from aerial parts. In the developed method, a C18 column was used. The mobile phase was composed of acetonitrile and water (45:55), whereas the method parameters included mobile phase flow rate at 0.8 mL/min, oven temperature at 40°C, and monitoring at 205 nm. In the validation, the parameters of selectivity, linearity, precision, accuracy, robustness, limits of detection and quantification were evaluated. As a result, the developed method is in accordance with the guidelines f or validation of analytical methods and presented satisfactory chromatographic parameters for YAN determination. Thus, the present analytical methodology can be applied in the quality control of O. duckei raw materials.
Ocotea duckei , conocida como Louro - de - cheiro, pertenece a la familia Lauraceae y presenta la yangambina lignoide (YAN) como principal marcador vegetal. Este trabajo tuvo como objetivo desarrollar y val idar un método analítico por cromatografía líquida de alta resolución para la cuantificación de YAN. La muestra utilizada fue el extracto etanólico crudo (EEC) obtenido de partes aéreas. En el método desarrollado se utilizó una columna C18. La fase móvil c onsistió en acetonitrilo y agua (45:55), mientras los parámetros del método incluyeron el caudal de la fase móvil a 0,8 m L /min, la temperatura del horno a 40°C y la monitorización a 205 nm. En la validación se evaluaron los parámetros de selectividad, line alidad, precisión, exactitud, robustez, límites de detección y cuantificación. Como resultado, el método desarrollado está de acuerdo con las pautas para la validación de métodos analíticos y presentó parámetros cromatográficos satisfactorios para la deter minación de YAN. Por lo tanto, la presente metodología analítica se puede aplicar en el control de calidad de las materias primas de O. duckei.
Subject(s)
Plant Extracts/chemistry , Chromatography, High Pressure Liquid/methods , Ocotea/chemistry , EthanolABSTRACT
Cladosporium carrionii is considered the most important pathogenic species of genus because of the numerous cases of disease which causes in the world. Due to its antifungal resistance, these fungal infections are difficult to treat. Given the broad biological activity displayed by natural products, essential oils obtained from plants are often investigated to determine their antimicrobial activity. Aims: Therefore, we identified components of Melissa officinalis L. essential oil, investigating in vitro antifungal activity against strains of C. carrionii. Methodology: Identification of the chemical composition of the oil was performed by gas chromatography coupled to a mass spectrometer (GC-MS). The antifungal activity of M. officinalis L. essential oil was investigated against 08 strains of C. carrionii determining minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC), effects on mycelial growth, and conidial germination. Results: The GC-MS results revealed 4 major components; geranial (52%), citral (38.90%), trans- β-caryophyllene (1.22%), and germacrene D (0.84%). M. officinalis L. essential oil inhibited the growth of all (100%) of the strains of C. carrionii tested. The MIC and MFC were established at 256 μg/ml. Inhibition of radial mycelial growth began at 128 μg/ml (MIC/2), and at both 2 x MIC and 4 x MIC the inhibition was complete. We also observed significant conidial germination inhibition at all concentrations when compared to the control (P<0.05). The inhibition increased with concentration so that at 2 x MIC complete (100%) conidia germination inhibition was observed. Conclusion: Finally, our study results point to M. officinalis L. essential oil as a potential antifungal agent against C. carrionii.