ABSTRACT
Our objective was to study the effect of dorzolamide on corneal hydration in an 18-week controlled experiment using ultrasonic pachymetry. Twenty-eight male rabbits were divided randomly into four groups. The 7 rabbits in each group received eye drops containing either 2 percent (w/v) dorzolamide or placebo in their right eye, or in their left eye. The 2 percent dorzolamide rabbits were treated every 8 h. Fellow eyes are defined as eyes which did not receive either dorzolamide or placebo. The study was blind for both the person who applied the drug and the one who performed the pachymetry. The effect of treatments is reported on the basis of the percentage of pachymetric variation compared to the measurement made before drug application. There was no significant difference (P = 0.061) in pachymetric variation between dorzolamide (-4.42 ± 11.71 percent) and placebo (2.48 ± 9.63 percent). However, there was a significant difference (P = 0.0034) in pachymetric variation between the dorzolamide fellow eyes (-7.56 ± 10.50 percent) and the placebo (-4.42 ± 11.71 percent). In conclusion, dorzolamide did not increase the corneal thickness in rabbits.
Subject(s)
Animals , Male , Rabbits , Carbonic Anhydrase Inhibitors/pharmacology , Cornea/drug effects , Sulfonamides/pharmacology , Thiophenes/pharmacology , Administration, Topical , Carbonic Anhydrase Inhibitors/administration & dosage , Cornea , Random Allocation , Sulfonamides/administration & dosage , Thiophenes/administration & dosageABSTRACT
Important biological and clinical features of malignancy are reflected in its transcript pattern. Recent advances in gene expression technology and informatics have provided a powerful new means to obtain and interpret these expression patterns. A comprehensive approach to expression profiling is serial analysis of gene expression (SAGE), which provides digital information on transcript levels. SAGE works by counting transcripts and storing these digital values electronically, providing absolute gene expression levels that make historical comparisons possible. SAGE produces a comprehensive profile of gene expression and can be used to search for candidate tumor markers or antigens in a limited number of samples. The Cancer Genome Anatomy Project has created a SAGE database of human gene expression levels for many different tumors and normal reference tissues and provides online tools for viewing, comparing, and downloading expression profiles. Digital expression profiling using SAGE and informatics have been useful for identifying genes that have a role in tumor invasion and other aspects of tumor progression
Subject(s)
Humans , Computational Biology , Databases as Topic , Gene Expression Profiling , Gene Library , Neoplasms , Antigens, Neoplasm , Expressed Sequence Tags , Genetic MarkersABSTRACT
We have developed a software called pp-Blast that uses the publicly available Blast package and PVM (parallel virtual machine) to partition a multi-sequence query across a set of nodes with replicated or shared databases. Benchmark tests show that pp-Blast running in a cluster of 14 PCs outperformed conventional Blast running in large servers. In addition, using pp-Blast and the cluster we were able to map all human cDNAs onto the draft of the human genome in less than 6 days. We propose here that the cost/benefit ratio of pp-Blast makes it appropriate for large-scale sequence analysis. The source code and configuration files for pp-Blast are available at http://www.ludwig.org.br/biocomp/tools/pp-blast
Subject(s)
Humans , Database Management Systems , Genome, Human , Internet , User-Computer Interface , Computing Methodologies , Cost-Benefit Analysis , Database Management Systems , Time FactorsABSTRACT
The artificially induced rat deciduoma serves as a model to study cellular changes associated with implantation in the endometrium. The stromal cells differentiate to form two types of decidual cells and are restricted to specific anatomical sites of the uterus. Programmed cell death starts in the antimesometrial area and expression of glutathione-S-transferase, an antioxidant enzyme, enhances in these cells as the deciduoma enters the regressive phase. The enzyme activity is significantly high compared with that of mesometrial decidual cells. Similarly, lipid peroxide content of antimesometrial decidual cells is high during this phase. DNA fragmentation, a feature of cells undergoing programmed cell death, is initiated in the antimesometrial area during regression of deciduoma.
Subject(s)
Animals , Apoptosis , Placenta/cytology , Rats , Rats, WistarABSTRACT
Enteric hyperoxaluria manifests due to hyperabsorption of dietary oxalate, secondary to a variety of chronic gastrointestinal disorders. The potential use of chitosan immobilized oxalate oxidase-catalase conjugate to deplete the oxalate content of food materials, while they are in the digestive tract has been evaluated by treating rat stomach chyme with such an enzyme preparation. Oxalate oxidase, obtained from beet stem, was adsorbed on chitosan along with catalase and then cross linked with glutaraldehyde to stabilize the derivative. This chemical modification of oxalate oxidase brought about a shift in its optimal pH from 4.2 to 3.8 with a marginal increase in its K(m). Compared to native enzyme, the modified oxalate oxidase exhibited increased storage stability, higher thermal stability and enhanced resistance to proteolytic digestion and heavy metal inactivation. These improved properties of the immobilized oxalate oxidase possibly render it suitable for oral administration under hyperoxaluric conditions.
Subject(s)
Animals , Catalase/metabolism , Chitin/analogs & derivatives , Chitosan , Enzymes, Immobilized , Gastrointestinal Contents , Hyperoxaluria/metabolism , Oxalates/metabolism , Oxidoreductases/metabolism , RatsABSTRACT
Extracellular matrix-degrading enzymes have a very important role in many normal and pathological processes. Members of the matrix metalloproteinase and plasminogen activator families are the major modulators of extracellular matrix degradation. Here, we discuss some topics about these enzymes giving special attention to the transcriptional and extracellular regulation of their expression.
Subject(s)
Humans , Animals , Extracellular Matrix/enzymology , Metalloproteases/metabolism , Blotting, Northern , Metalloproteases/physiology , Plasminogen Activators , Urokinase-Type Plasminogen ActivatorSubject(s)
Humans , Antigens, Human Platelet/immunology , Autoantibodies , Autoimmune Diseases , Epitopes , Platelet Membrane Glycoproteins/immunology , Purpura, Thrombocytopenic, Idiopathic/immunology , Receptors, Fibronectin , Amino Acid Sequence , Antibody Specificity , Integrins , Molecular Sequence DataABSTRACT
Utilizando-se de medidas de intervalos anatomicos do olho e fenda palpebral, os autores analisam a magnitude dos erros experimentais cometidos na obtencao das mesmas e propoe um metodo de avaliacao da pericia do leitor escolhido para tais mensuracoes
Subject(s)
Eye , Weights and MeasuresABSTRACT
Um estudo comparativo das medidas da posicao ocular obtidas com os metodos de Hirschberg e de Krimsky foi feito em 50 olhos de pacientes estrabicos submetidos a anestesia geral. Conclui-se que, tolerando-se pequenas diferencas, as leituras fornecidas pelos dois metodos sao equivalentes