Rat thyroid graft transplantation after cryopreservation with scintigraphic standardization for an experimental study

Abstract Purpose: Budget cuts among other factors undermine the use of state-of-the-art equipment by many research groups. This doesn't mean that their scientific data are not reliable or top-notch. Resort to adaptations is a recurrent need in their reality. The aim of this study was to assess whether scintigraphy with 99mTcO4 is effective in evaluating the functionality of thyroid grafts after cryopreservation in rats. Material and methods: 24 rats were randomly distributed into 3 groups: Control Group (CG), without surgical procedure, Hypothyroidism Group (HTG), submitted to total thyroidectomy, and Transplanted Group (TG), with total thyroidectomy and cryopreservation of the thyroid gland for 7 days followed by grafting of a thyroid lobe. A protocol using a gamma camera imaging was conducted fourteen weeks after transplantation, and the whole body 99mTc, focusing on the topic of heterotopic thyroid uptake was evaluated. Results: The images acquired had good quality with no noise and artifacts that could jeopardize its analysis. On the 14th day, HTG displayed no thyroid uptake, and the TG had a clear uptake of the thyroid graft in the topography of the biceps femoris muscle. Presented data also showed that both equipment spatial resolution and alignment (4.375 mm) did not interfere with the physiological uptake of 99mTc by the thyroid graft. Conclusion: The viability and functionality of cryopreserved thyroid autotransplantation in rats who underwent total thyroidectomy were successfully accessed by the scintigraphy protocol developed. HIGHLIGHTS Adaptations in validated methods are a recurrent trend due to limited budgets which does not diminish their functionality. Scintigraphy with 99mTcO4 is effective in evaluating the functionality of thyroid grafts after cryopreservation in rats. It is possible to adapt human SPECT for other animals for clinical and research purposes.

Cenário Disfuncional dos Principais Componentes Responsáveis pelo Equilíbrio do Trânsito de Cálcio Miocárdico na Insuficiência Cardíaca Induzida por Estenose Aórtica / The Dysfunctional Scenario of the Major Components Responsible for Myocardial Calcium Balance in Heart Failure Induced by Aortic Stenosis

Resumo Fundamento O remodelamento cardíaco patológico se caracteriza por disfunção diastólica e sistólica, levando à insuficiência cardíaca. Neste contexto, o cenário disfuncional do trânsito de cálcio miocárdico (Ca2+) tem sido pouco estudado. Um modelo experimental de estenose aórtica tem sido extensamente utilizado para aprimorar os conhecimentos sobre os principais mecanismos do remodelamento patológico cardíaco. Objetivo Entender o processo disfuncional dos principais componentes responsáveis pelo equilíbrio do cálcio miocárdico e sua influência sobre a função cardíaca na insuficiência cardíaca induzida pela estenose aórtica. Métodos Ratos Wistar de 21 dias de idade foram distribuídos em dois grupos: controle (placebo; n=28) e estenose aórtica (EaO; n=18). A função cardíaca foi analisada com o ecocardiograma, músculo papilar isolado e cardiomiócitos isolados. No ensaio do músculo papilar, SERCA2a e a atividade do canal de Ca2+ do tipo L foram avaliados. O ensaio de cardiomiócitos isolados avaliou o trânsito de cálcio. A expressão proteica da proteínas do trânsito de cálcio foi analisada com o western blot. Os resultados foram estatisticamente significativos quando p <0,05. Resultados Os músculos papilares e cardiomiócitos dos corações no grupo EaO demonstraram falhas mecânicas. Os ratos com EaO apresentaram menor tempo de pico do Ca2+, menor sensibilidade das miofibrilas do Ca2+, prejuízos nos processos de entrada e recaptura de cálcio pelo retículo sarcoplasmático, bem como disfunção no canal de cálcio do tipo L (CCTL). Além disso, os animais com EaO apresentaram maior expressão de SERCA2a, CCTL e trocador de Na+/Ca2+. Conclusão Insuficiência cardíaca sistólica e diastólica devido à estenose aórtica supravalvular acarretou comprometimento da entrada de Ca2+ celular e inibição da recaptura de cálcio pelo retículo sarcoplasmático devido à disfunção no CCTL e SERCA2a, assim como mudanças no trânsito de cálcio e na expressão das principais proteínas responsáveis pela homeostase de Ca2+ celular.

Abstract Background Maladaptive cardiac remodelling is characterized by diastolic and systolic dysfunction, culminating in heart failure. In this context, the dysfunctional scenario of cardiac calcium (Ca2+) handling has been poorly studied. An experimental model of aortic stenosis has been extensively used to improve knowledge about the key mechanisms of cardiac pathologic remodelling. Objective To understand the dysfunctional process of the major components responsible for Ca2+ balance and its influence on cardiac function in heart failure induced by aortic stenosis. Methods Male 21-day-old Wistar rats were distributed into two groups: control (sham; n= 28) and aortic stenosis (AoS; n= 18). Cardiac function was analysed by echocardiogram, isolated papillary muscle, and isolated cardiomyocytes. In the papillary muscle assay, SERCA2a and L-type Ca2+ channel activity was evaluated. The isolated cardiomyocyte assay evaluated Ca2+ handling. Ca2+ handling protein expression was analysed by western blot. Statistical significance was set at p <0.05. Results Papillary muscles and cardiomyocytes from AoS hearts displayed mechanical malfunction. AoS rats presented a slower time to the Ca2+ peak, reduced Ca2+ myofilament sensitivity, impaired sarcoplasmic reticulum Ca2+ influx and reuptake ability, and SERCA2a and L-type calcium channel (LTCC) dysfunction. Moreover, AoS animals presented increased expression of SERCA2a, LTCCs, and the Na+/Ca2+ exchanger. Conclusion Systolic and diastolic heart failure due to supravalvular aortic stenosis was paralleled by impairment of cellular Ca2+ influx and inhibition of sarcoplasmic reticulum Ca2+ reuptake due to LTCC and SERCA2a dysfunction, as well as changes in Ca2+ handling and expression of the major proteins responsible for cellular Ca2+ homeostasis.

Mesenchymal stem cells surpass the capacity of bone marrow aspirate concentrate for periodontal regeneration

Abstract Regenerative approaches using mesenchymal stem cells (MSCs) have been evaluated to promote the complete formation of all missing periodontal tissues, e.g., new cementum, bone, and functional periodontal ligaments. MSCs derived from bone marrow have been applied to bone and periodontal defects in several forms, including bone marrow aspirate concentrate (BMAC) and cultured and isolated bone marrow mesenchymal stem cells (BM-MSCs). This study aimed to evaluate the periodontal regeneration capacity of BMAC and cultured BM-MSCs in the wound healing of fenestration defects in rats. Methodology: BM-MSCs were obtained after bone marrow aspiration of the isogenic iliac crests of rats, followed by cultivation and isolation. Autogenous BMAC was collected and centrifuged immediately before surgery. In 36 rats, fenestration defects were created and treated with suspended BM-MSCs, BMAC or left to spontaneously heal (control) (N=6). Their regenerative potential was assessed by microcomputed tomography (µCT) and histomorphometry, as well as their cell phenotype and functionality by the Luminex assay at 15 and 30 postoperative days. Results: BMAC achieved higher bone volume in 30 days than spontaneous healing (p<0.0001) by enhancing osteoblastic lineage commitment maturation, with higher levels of osteopontin (p=0.0013). Defects filled with cultured BM-MSCs achieved higher mature bone formation in early stages than spontaneous healing and BMAC (p=0.0241 and p=0.0143, respectively). Moreover, significantly more cementum-like tissue formation (p<0.0001) was observed with new insertion of fibers in specimens treated with BM-MSCs within 30 days. Conclusion: Both forms of cell transport, BMAC and BM-MSCs, promoted bone formation. However, early bone formation and maturation were achieved when cultured BM-MSCs were used. Likewise, only cultured BM-MSCs were capable of achieving complete periodontal regeneration with inserted fibers in the new cementum-like tissue.

Effect of implant crestal position on primary stability before and after loading: an in vitro study / Efeito do posicionamento crestal do implante na estabilidade primária antes e após carga: um estudo in vitro

Introduction: Primary stability is one of the goals of modern implant dentistry and if achieved, reduces treatment time for prosthetic rehabilitation and the number of interventions made in patients mouth. Several companies state as protocol for connical conection implants, a subcrestally positioning. Objective: This in vitro study aimed to evaluate the effect of placing a conical connection implant equicrestally and subcrestally on static and loading condition in two types of bone density. Material and method: A total of 200 bone cylinders were extracted from femur of pigs, standardized by means of x-rays and computerized microtomography scan (microCT) and separated in low and high density specimens. The implants were placed on the center of the bone cylinders and were evaluated before and after loading by means of microCT and histomorphometry. Result: The results showed that placing the evaluated implant subcrestally provided better primary stability and performance on static and loading situations on low and high density bone. Conclusion: Placing implant subcrestally improve primary stability outcomes under loading and static situations.

Introdução: A estabilidade primária é um dos objetivos da implantodontia moderna e, caso atingida, reduz o tempo de tratamento para a reabilitação protéticas e o número de intervenções realizadas. Diversas empresas preconizam a posição subcrestal no uso de implantes com conexão cônica interna. Objetivo: Este estudo in vitro avaliou o efeito do posicionamento de implantes de conexão conica interna sub e equicrestal sob condições estáticas e em função, considerando dois tipos de densidades ósseas. Material e método: um total de 200 espécimes de osso extraído do femur de suínos e padronizados por meio de radiografias e microtomografias computadorizadas foram separados em densidade alta e baixa. Implantes foram instalados no centro dos especimes e for a avaliados por meio de microCT e histomorfometria. Resultado: Os resultados demonstraram que a colocação de implante subcrestalmente promoveu melhor estabilidade primária e performance em todas as situações, irrespectivamente à densidade óssea. Conclusão: A colocação de implantes subcrestalmente melhora a estabilidade primária em todas as situações, sendo indicada quando da utilização de conexões cônicas internas.

Anatomy of the nerves, vessels, and muscular compartments of the forearm, as revealed by high-resolution ultrasound. Part 1: overall structure and forearm compartments

Abstract In recent decades, high-resolution ultrasound (HRUS) has revolutionized the morphological and structural evaluation of peripheral nerves and muscles, revealing details of the internal structure of the neural fascicles and muscle architecture. Applications range from diagnostics to interventional procedures. The anatomy of the forearm region is complex, with several muscles and an extensive network of vessels and nerves. To guarantee the success of the evaluation by HRUS, knowledge of the normal anatomy of the region is essential. The aim of these two companion articles is to present the normal anatomy of the nerves and compartments of the forearm, as revealed by HRUS, as well as the relationships between the main vessels and nerves of the region. Part 1 aims to review the overall structure of nerves, muscles and tendons, as seen on HRUS, and that of the forearm compartments. We present a practical approach, with general guidelines and tips on how best to perform the study. Part 2 is a pictorial essay about compartment vascularization and cutaneous innervation. Knowledge of the normal anatomy of the forearm improves the technical quality of the examinations, contributing to better diagnoses, as well as improving the performance and safety of interventional procedures.

Anatomy of the nerves, vessels, and muscular compartments of the forearm, as revealed by high-resolution ultrasound. Part 2: vascularization of compartments and cutaneous innervation

Abstract In recent decades, high-resolution ultrasound (HRUS) has revolutionized the morphological and structural evaluation of peripheral nerves and muscles, revealing details of the internal structure of the neural fascicles and muscle architecture. Applications range from diagnostics to interventional procedures. The anatomy of the forearm region is complex, with several muscles and an extensive network of vessels and nerves. To guarantee the success of the evaluation by HRUS, knowledge of the normal anatomy of the region is essential. The aim of these two companion articles is to present the normal anatomy of the nerves and compartments of the forearm, as revealed by HRUS, as well as the relationships between the main vessels and nerves of the region. Part 1 aims to review the overall structure of nerves, muscles and tendons, as seen on HRUS, and that of the forearm compartments. We present a practical approach, with general guidelines and tips on how best to perform the study. Part 2 is a pictorial essay about compartment vascularization and cutaneous innervation. The relationships between arteries, satellite veins and nerves, as well as the relationship between cutaneous nerves and superficial veins, are demonstrated. Knowledge of the normal anatomy of the forearm improves the technical quality of the examinations, contributing to better diagnoses, as well as improving the performance and safety of interventional procedures.

Pensar, gostar e considerar importante a prática de atividade física aumentam as chances de ser ativo no lazer? / Do thinking, enjoying, and considering important the practice of physical activity increase the chances of being active at leisure?

RESUMO: Embora reconhecida como comportamento de extrema importância na saúde da população, a prática de atividade física regular ainda é um grande desafio. Objetivo: verificar o nível de atividade física no lazer e possíveis fatores correlatados em uma comunidade acadêmica do nordeste brasileiro. Metodologia: trata-se de um estudo transversal, constituído por discentes, docentes e técnicos administrativos, utilizou-se como instrumento "Mapa de Atividade Física e Saúde-MAFIS", realizou-se estatística descritiva, teste de Qui quadrado, com posterior montagem do modelo de regressão logística. Resultados: 52,1% atingiram as recomendações de atividade física (AF), os homens apresentam 65,3% (OR: 1,65; IC95%: 1,29-2,11) mais chance de serem ativos, quem gosta de AF tem duas (OR: 2,02; IC95%: 0,82-4,96) a 4,8 (OR: 4,84; IC95%: 1,99-11,8) vezes mais chance de praticar AF, achar importante fazer AF (OR: 2,23; IC95%: 0,48-10,4) e menos dias sentado aumenta de 48,3% (OR: 1,48; IC95%: 1,032- 2,130) a 53,3% (OR: 1,53; IC95%: 1,09-2,13) a chance de fazer AF e quem pensa em fazer AF (OR: 0,362; IC: 0,203-0,645) tem menos chance de ser ativo. Conclusão: Gostar, considerar importante e menos dias sentados aumentam as chances de fazer atividade física, já pensar sempre em fazer, diminui a chance de ser ativo no lazer. (AU)

ABSTRACT: Although recognized as extremely important behavior in the health of the population, the practice of a regular physical activity is still a major challenge. Aim: verifying the level of leisure-time physical activity and possible correlated factors in an academic community in northeastern Brazil. Materials and methods: this is a cross-sectional study, consisting of stu-dents, professors, and administrative technicians. The instrument "Map of Physical Activity and Health-MAFIS" was used, as well as descriptive statistics, Chi-square test, with the subsequent assembly of the logistic regression model. Results: 52.1% reached the recommendations for physical activity (PA), men were 65.3% (OR: 1.65; 95% CI: 1.29 - 2.11) more likely to be active, those who like PA were two (OR: 2.02; 95% CI: 0.82- 4.96) to 4.8 (OR: 4.84; 95% CI: 1.99-11.8) times more likely to practice PA, finding it important to do AF (OR: 2.23; 95% CI: 0.48-10.4) and spending fewer days sitting increased by 48.3% (OR: 1.48; 95% CI: 1,032 - 2,130) to 53.3% (OR: 1 , 53; 95% CI: 1.09 - 2.13) the chance of doing PA and those who think about doing PA (OR: 0.362; CI: 0.203 - 0.645) are less likely to be active. Conclusion:Enjoying, considering important, and spending fewer days sitting increase the chances of doing physical activity, on the other hand, just thinking about doing it decreases the chances of being active at leisure. (AU)

Atividade física na prevenção de doenças crônicas não transmissíveis em homens / Physical activity in the prevention of chronic noncommunicable diseases in men

RESUMO: Introdução: A promoção da atividade física (AF) é uma das prioridades das políticas públicas de atenção básica à saúde, especialmente para o público masculino. Objetivo: Avaliar a associação da prática de atividade física com o estilo de vida e presença doenças crônicas não transmissíveis (DCNTs) em usuários do sexo masculino de Unidade Básica de Saúde (UBS). Métodos: Participaram do estudo 119 homens na faixa etária de 20 a 59 anos. A associação entre a prática de atividade física com variáveis sociodemográficas, etilismo, tabagismo, uso de medicamento, presença de doenças crônicas não transmissíveis e conhecimento da política de saúde do homem foi realizada por Regressão Logística com um α=5%. Resultados: A prevalência de atividade física foi de 58,8%, entretanto 36,7% dos participantes faziam uso de medicações diariamente e 70,6% não conhecem a Política Nacional de Atenção Integral à Saúde do Homem. Além disso, fazer atividade física demonstrou como um fator de proteção contra as doenças crônicas não transmissíveis (OR = 2,97; IC 95% 1,03 ­ 8,55; p = 0,043). Conclusão: A prática de atividade física em homens, independentemente da idade, tabagismo e etilismo foi associada à prevenção de doenças crônicas não transmissíveis.(AU)

ABSTRACT: Introduction: The promotion of physical activity (PA) is one of the priorities of public policies for primary health care, especially for the male audience. Objective: To evaluate the association between physical activity and life-style and presence of chronic noncommunicable diseases (NCDs) in male users of the Basic Health Unit (BHU). Methods: 119 men, aged between 20 and 59 years, participated in the study. The association between the practice of physical activity with sociodemographic variables, alcoholism, smoking, use of medication, presence of chronic noncommunicable diseases and knowledge of men's health policy was carried out by Logistic Regression with an α= 5%. Results: The prevalence of physical activity was 58.8%, however 36.7% of the participants used medica-tions daily and 70.6% did not know the National Policy for Comprehensive Attention to Men's Health. In addition, physical activity proved to be a protective factor against chronic non-communicable diseases (OR= 2,97; IC 95% 1,03 ­ 8,55; p = 0,043). Conclusion: The practice of physical activity in men, regardless of age, smoking and alcohol consumption was associated with the prevention of chronic noncommunicable diseases. (AU)

A Redução do Colágeno Tipo I está Associada ao Aumento da Atividade da Metaloproteinase-2 e da Expressão Proteica de Leptina no Miocárdio de Ratos Obesos / Decreased Collagen Type I is Associated with Increased Metalloproteinase-2 Activity and Protein Expression of Leptin in the Myocardium of Obese Rats

Resumo Fundamento A obesidade é um fator de risco para complicações médicas, incluindo o sistema cardiovascular. Há informações limitadas sobre o colágeno no coração obeso. Nosso estudo anterior demonstrou uma redução dos níveis proteicos de colágeno miocárdico tipo I em ratos obesos alimentados com uma dieta com alto teor de gordura durante 34 semanas. No entanto, os mecanismos responsáveis pelos níveis baixos não estão completamente elucidados. Objetivo O objetivo deste estudo foi testar a hipótese de que a redução do colágeno tipo I está associada ao aumento da atividade da metaloproteinase-2 (MMP-2), a qual está ligada à elevação de leptina no miocárdio de ratos obesos. Métodos Ratos Wistar machos com 30 dias de idade foram randomizados em dois grupos: controle (dieta padrão) e obeso (dieta com alto teor de gordura), e alimentados durante 34 semanas. Foram avaliados as características gerais dos animais e os perfis metabólicos e endócrinos. Foram avaliados as expressões proteicas miocárdicas de colágeno tipo I, leptina e inibidores teciduais de metaloproteinases (TIMP), bem como a atividade da MMP-2. O teste de correlação de Pearson foi aplicado para determinar as associações entre variáveis. O nível de significância foi de 5%. Resultados Os animais obesos apresentaram índice de adiposidade mais elevado em comparação ao controle. Foram observadas comorbidades como intolerância à glicose, hiperinsulinemia, resistência à insulina, hiperleptinemia e hipertensão nos ratos obesos. A obesidade reduziu o colágeno tipo I, TIMP-1 e TIMP-2, e aumentou a leptina e a MMP-2 no miocárdio. Houve uma correlação negativa entre o colágeno tipo I e a MMP-2 e uma correlação positiva entre a leptina e a MMP-2. Conclusão Foi confirmada a hipótese de que a redução do colágeno tipo I está associada ao aumento da atividade da MMP-2 e da expressão de leptina no miocárdio de ratos obesos. (Arq Bras Cardiol. 2020; 115(1):61-70)

Abstract Background Obesity is a risk factor for medical complications, including the cardiovascular system. There is limited information on collagen in the heart in obesity. Our previous study showed decreased protein levels of myocardial collagen type I in obese rats fed a high-fat diet for 34 weeks. However, the mechanisms responsible for low levels are not fully elucidated. Objective The purpose of this study was to test the hypothesis that the reduction in collagen type I is associated with increased metalloproteinase-2 (MMP-2) activity, which is linked to elevated leptin in the myocardium of obese rats. Methods Thirty-day-old male Wistar rats were randomized into two groups, control (standard diet) and obese (high-fat diet), and fed for 34 weeks. The general animal characteristics and metabolic and endocrine profiles were evaluated. Myocardial protein expressions of collagen I, leptin, tissue inhibitors of metalloproteinases (TIMP), and MMP-2 activity were assessed. Pearson correlation was employed to determine the associations between variables. The level of significance was 5%. Results The obese animals had increased adiposity index compared to control. Comorbidities such as glucose intolerance, hyperinsulinemia, insulin resistance, hyperleptinemia, and hypertension were observed in obese rats. Obesity reduced collagen I, TIMP-1, and TIMP-2, and it increased leptin and MMP-2 in the myocardium. There was a negative correlation between collagen I and MMP-2 and a positive correlation between leptin and MMP-2. Conclusion The hypothesis was confirmed; the reduction in collagen type I is associated with increased MMP-2 activity and leptin expression in the myocardium of obese rats. (Arq Bras Cardiol. 2020; 115(1):61-70)

Brazilian version of the Self-Estimated Functional Inability because of Pain questionnaire for musculoskeletal injuries relating to dance and sport: translation and cross-cultural adaptation

ABSTRACT BACKGROUND: Self-Estimated Functional Inability because of Pain (SEFIP) is a questionnaire specifically designed to measure musculoskeletal pain or discomfort. OBJECTIVE: To perform translation and cross-cultural adaptation of the SEFIP for dancers (SEFIP-dance), for use in Brazilian Portuguese. In addition, as a secondary objective, we adapted the translated version of SEFIP-dance for use among athletes or exercise practitioners (SEFIP-sport). DESIGN AND SETTING: Questionnaire translation and cross-cultural adaptation study conducted at a public university. METHODS: The Brazilian version of the SEFIP-dance questionnaire was developed following the processes of translation (involving two translators with Brazilian Portuguese as their mother tongue and fluency in English), backtranslation (involving two translators with English as their mother tongue and fluency in Brazilian Portuguese), committee review and pre-testing. SEFIP-sport was developed following the processes of content and face validation. RESULTS: SEFIP-dance was applied to 30 dancers, of mean age 22.38 years (standard deviation [SD] = 3.41), among whom 14 were men (46.66%). The participants understood 100% of the SEFIP-dance items and alternatives. SEFIP-sport was applied to 30 athletes or physical exercise practitioners, of mean age 25.09 years (SD = 8.93), among whom 25 were men (86.33%). The participants understood 100% of the ­SEFIP-sport items and alternatives. CONCLUSION: The Brazilian Portuguese versions of SEFIP-dance, translated and cross-culturally adapted for dancers, and SEFIP-sport, adapted for athletes or physical exercise practitioners, were shown to have adequate levels of understanding.

Inflammatory lesions and brain tumors: is it possible to differentiate them based on texture features in magnetic resonance imaging?

Neuroimaging strategies are essential to locate, to elucidate the etiology, and to the follow up of brain disease patients. Magnetic resonance imaging (MRI) provides good cerebral soft-tissue contrast detection and diagnostic sensitivity. Inflammatory lesions and tumors are common brain diseases that may present a similar pattern of a cerebral ring enhancing lesion on MRI, and non-enhancing core (which may reflect cystic components or necrosis) leading to misdiagnosis. Texture analysis (TA) and machine learning approaches are computer-aided diagnostic tools that can be used to assist radiologists in such decisions. Methods: In this study, we combined texture features with machine learning (ML) methods aiming to differentiate brain tumors from inflammatory lesions in magnetic resonance imaging. Retrospective examination of 67 patients, with a pattern of a cerebral ring enhancing lesion, 30 with inflammatory, and 37 with tumoral lesions were selected. Three different MRI sequences and textural features were extracted using gray level co-occurrence matrix and gray level run length. All diagnoses were confirmed by histopathology, laboratorial analysis or MRI. Results: The features extracted were processed for the application of ML methods that performed the classification. T1-weighted images proved to be the best sequence for classification, in which the differentiation between inflammatory and tumoral lesions presented high accuracy (0.827), area under ROC curve (0.906), precision (0.837), and recall (0.912). Conclusion: The algorithm obtained textures capable of differentiating brain tumors from inflammatory lesions, on T1-weghted images without contrast medium using the Random Forest machine learning classifier.(AU)

Modes of commuting to school among 5th and 6th grade schoolchildren / Modos de deslocamento para a escola em crianças do 5º e 6º ano de escolaridade

Abstract Active commuting (walking/cycling) is seen as a strategy to promote active behaviors in children's daily school life. This study aimed to analyze the modes of commuting and perceptions of children in relation to the possibility of active commuting to school according to sex. Overall, 101 students (M=11.39/SD=1.22) enrolled in the 5th and 6th grades from two public elementary schools in the state of Maranhão, Brazil, participated in this study. Data were collected through a questionnaire administered to students. The results showed that 21.8% of respondents had active commuting (19.8% on foot / 2% bicycle), without statistically significant differences; however, boys were more predisposed to walking and cycling in relation to girls. As positive justifications, respondents evidenced the 'home-school proximity' and 'fun/pleasure' provided during the journey, and 'distance' and 'traffic / insecurity' as barriers to the possibility of walking or cycling to school.

Resumo Vislumbra-se o deslocamento ativo (a pé/bicicleta) como uma estratégia de promoção de comportamentos ativos no cotidiano escolar de crianças. Este estudo objetivou analisar os modos de deslocamento e percepções de crianças em relação à possibilidade do deslocamento ativo para a escola de acordo com o sexo. Participaram 101 alunos ( x x=11,39/DP=1,22) do 5º e 6º ano de escolaridade de duas escolas públicas do estado do Maranhão,Brasil. Os dados foram coletados através de um questionário administrado aos alunos. Os resultados demonstraram que 21,8% dos inquiridos se deslocaram ativamente (19,8% a pé/2% bicicleta), sem diferenças estatisticamente significativas, contudo, os meninos apresentaram maior predisposição à caminhada e utilização da bicicleta em relação às meninas. Como justificativas positivas os inquiridos evidenciaram a 'Proximidade casa-escola' e 'Diversão/prazer' proporcionadas durante o trajeto e, a 'distância' e a 'insegurança trânsito/geral' como barreiras frente a possibilidade em realizar o percurso a pé ou de bicicleta para a escola.

Clinical attachment loss and molecular profile of inflamed sites before treatment

Abstract Objective: To monitor early periodontal disease progression and to investigate clinical and molecular profile of inflamed sites by means of crevicular fluid and gingival biopsy analysis. Methodology: Eighty-one samples of twenty-seven periodontitis subjects and periodontally healthy individuals were collected for the study. Measurements of clinical parameters were recorded at day −15, baseline and 2 months after basic periodontal treatment aiming at monitoring early variations ofthe clinical attachment level. Saliva, crevicular fluid and gingival biopsies were harvested from clinically inflamed and non-inflamed sites from periodontal patients and from control sites of healthy patients for the assessment of IL-10, MMP-8, VEGF, RANKL, OPG and TGF-β1 protein and gene expression levels. Results: Baseline IL-10 protein levels from inflamed sites were higher in comparison to both non-inflamed and control sites (p<0.05). Higher expression of mRNA for IL-10, RANK-L, OPG, e TGF-β1 were also observed in inflamed sites at day −15 prior treatment (p<0.05). After the periodontal treatment and the resolution of inflammation, seventeen percent of evaluated sites still showed clinically detectable attachment loss without significant differences in the molecular profile. Conclusions: Clinical attachment loss is a negative event that may occur even after successful basic periodontal therapy, but it is small and limited to a small percentage of sites. Elevated inflammation markers of inflamed sites from disease patients reduced to the mean levels of those observed in healthy subjects after successful basic periodontal therapy. Significantly elevated both gene and protein levels of IL-10 in inflamed sites prior treatment confirms its modulatory role in the disease status.

Exploring cortical bone density through the ultrasound integrated reflection coefficient / Densidade óssea cortical analisada pelo coeficiente de reflexão ultrassônica

ABSTRACT Objective: This work evaluates the relationship between ultrasonic reflection and bone density from fourteen cylindrical bovine cortical bone samples (3.0-cm thick). Methods: Twenty US reflection signals per sample were acquired along the bone surface (2.0-mm step). The Integrated Reflection Coefficient (IRC) from each signal was compared to Quantitative Computed Tomography (QCT). Results: Seven IRC and QCT curves presented Pearson's Correlation R-values above 0.5. For weak correlation curves, QCT and IRC showed similar trends in several segments. Conclusion: IRC was sensitive to bone density variation. Level of Evidence: Experimental Study, Investigating a Diagnostic Test.

RESUMO Objetivo: Este estudo avalia a relação entre a reflexão ultrassônica e a densidade óssea de 14 amostras cilíndricas de osso cortical bovino (3,0 cm de espessura). Métodos: Foi realizada a aquisição de 20 sinais de reflexão ultrassônica por amostra (passo de 2,0 mm), ao longo da superfície óssea. O Coeficiente de Reflexão Integrado (IRC) de cada sinal foi comparado por Tomografia Computadorizada Quantitativa (QCT). Resultados: Sete curvas de IRC e QCT apresentaram valor de Correlação R de Pearson acima de 0,5. Para curvas de correlação fraca, QCT e IRC apresentaram tendências semelhantes em vários segmentos. Conclusão: O IRC foi sensível à variação da densidade óssea. Nível de evidência: Estudo Experimental, Investigação de Exame Diagnóstico.

Doença crônica na infância e adolescência: vínculos da família na rede de atenção à saúde / Enfermedad crónica en la infancia y adolescencia: vínculos de la familia en la red de atención a la salud / Chronic disease in childhood andadolescence: family bondsin the healthcare network

RESUMO Objetivo: explicitar como se constroem os vínculos entre a família da criança/adolescente com doença crônica e os diferentes serviços da rede de atenção à saúde, tendo a Atenção Primária à Saúde como o centro organizador do cuidado. Método: estudo de abordagem qualitativa, realizado com dez familiares de crianças/adolescentes com diagnóstico de doença crônica entre maio e novembro de 2015. Para produzir o material empírico, utilizaram-se o mapa falante e o ecomapa, ambos adaptados neste estudo, em seus domicílios, localizados na área de abrangência de Unidades de Saúde da Família e na própria Unidade de Saúde da Família, conforme preferência de um familiar. Posteriormente, foram realizadas doze entrevistas semiestruturadas com profissionais de serviços da rede de atenção à saúde do município - duas Unidades de Saúde da Família e dois hospitais públicos. Os dados foram interpretados com base na análise de discurso de linha francesa. Resultados: o sentido de vínculo está relacionado à atenção dispensada pelos profissionais de saúde. A atenção explicitada nas marcas textuais analisadas é um conceito base, que pode ser parafraseado em seus vários sentidos, como: acessibilidade, resolutividade, responsabilização, humanização, empatia e expectativa satisfeita. Conclusão: é preciso que cada ponto de atenção da rede assistencial buscado pela família lhe seja uma referência ampla, concreta e duradoura, especialmente a Atenção Primária à Saúde, que deve cumprir seu papel de coordenadora do cuidado e fonte cuidadora.

RESUMEN Objetivo: explicitar cómo se construyen los vínculos entre la familia del niño/adolescente con enfermedad crónica y los diferentes servicios de la red de atención a la salud, teniendo la Atención Primaria a la Salud como el centro organizador del cuidado. Método: estudio de abordaje cualitativo, realizado con diez familiares de niños/adolescentes con diagnóstico de enfermedad crónica entre mayo y noviembre de 2015. Para producir el material empírico, se utilizaron el mapa hablante y el ecomapa, ambos adaptados en este estudio, en sus estudios en el área de cobertura de las Unidades de Salud de la Familia y en la propia Unidad de Salud de la Familia, según preferencia de un familiar. Posteriormente, se realizaron doce entrevistas semiestructuradas con profesionales de servicios de la red de atención a la salud del municipio - dos Unidades de Salud de la Familia y dos hospitales públicos. Los datos fueron interpretados con base en el análisis de discurso de línea francesa. Resultados: el sentido de vínculo está relacionado a la atención dispensada por los profesionales de salud. La atención explicitada en las marcas textuales analizadas es un concepto base, que puede ser parafraseado en sus diversos sentidos, como: accesibilidad, resolutividad, responsabilización, humanización, empatía y expectativa satisfecha. Conclusión: es necesario que cada punto de atención de la red asistencial buscado por la familia le sea una referencia amplia, concreta y duradera, especialmente la Atención Primaria a la Salud, que debe cumplir su papel de coordinadora del cuidado y fuente cuidadora.

ABSTRACT Objective: to explain how the bonds between the family of the child/adolescent with chronic disease are created and the different services of the health care network, having the Primary Health Care as the organizing center for care. Method: qualitative approach study, carried out with ten family members of children/adolescents diagnosed with a chronic disease between May and November 2015. In order to produce the empirical material, the talking map and the ecomap have been used, both adapted in this study, in their homes, located in the area covered by the Family Health Units and in the Family Health Unit itself, according to a family member's preference. Subsequently, twelve semi-structured interviews were carried out with professionals of the city's healthcare network - two Family Health Units and two public hospitals. The data were interpreted based on the French line discourse analysis. Results: the sense of bonding is related to the care and attention provided by health professionals. The care presented in the textual marks analyzed is a basic concept, which can be paraphrased in its several meanings, such as: accessibility, resoluteness, accountability, humanization, empathy and satisfied expectation. Conclusion: it is necessary that each care point of the healthcare network sought by the family is a broad, concrete and lasting reference, especially the Primary Health Care, which should fulfill its role of a care coordinator and caregiver source.

Genetic divergences between Spondias mombin (Anacardiaceae) genotypes found through morphological traits / Divergencia genética entre genotipos de Spondias mombin (Anacardiaceae) según caracteres morfológicos

Abstract The species Spondias mombin, is native to the Amazonian region. Since these trees' cultivation is incipient, their exploitation is done through extraction techniques. The aim of the present study was to assess the genetic divergences between S. mombin genotypesand to quantify the relative contribution from 12 morphological traits of the species' fruits and seeds, as well as to collect data able to subsidize future research on the species conservation and domestication. 60 genotypes were assessed in total, and ten fruits of each genotype were analyzed. Eight descriptors were used for fruit characterization, namely: fruit mass, pulp weight, volume, length, width, thickness, total soluble solids content and hydrogenionic potential. The seed descriptors were mass, length, width and thickness. The data were assessed through the principal components and groupings by applying the UPGMA and Tocher methods. They were analyzed in the GENES software, based on the dissimilarity matrix (Euclidean distance average). The analysis applied to the principal components showed that the first three components explained 83 % accumulated variation. The main traits contributing to the genotype discrimination were fruit width, fruit pulp weight, pH, seed length and thickness, and the most responsive traits to S. mombin genotype selection. The features fruit mass, seed width, fruit thickness, fruit volume, fruit length, seed mass and total soluble solid content presented the smallest contribution to diversity. The grouping methods UPGMA and Tocher evidenced genetic divergence between the analyzed genotypes. Genotypes 37 and 41 were more divergent than the others, what makes them promising for crossings in future genetic enhancement programs focused on the species' domestication.

Resumen Spondias mombin es un árbol nativo de la región amazónica; su explotación se produce de manera extractiva, siendo aún incipiente el cultivo de la especie. El objetivo de este estudio fue evaluar la divergencia genética entre genotipos de S. mombin y cuantificar la contribución relativa de 12 características morfológicas de frutos y semillas, para obtener datos que permitan investigaciones futuras relativas a la conservación y domesticación de la especie. Se evaluaron 60 genotipos; de cada uno se analizaron diez frutos. Los descriptores utilizados para la caracterización de los frutos fueron ocho: masa del fruto, peso de la pulpa, volumen, longitud, anchura, espesor, contenido total de sólidos solubles y pH; los descriptores de semillas fueron cuatro: masa, longitud, anchura y espesor. Los datos se evaluaron por medio de componentes principales y agrupaciones obtenidas por métodos UPGMA y Tocher, de la matriz de disimilitud (distancia euclideana media) con ayuda del programa GENES. A través del análisis de los componentes principales, se verificó que los tres primeros componentes explican 83.63 % de la variación acumulada. Las características que más contribuyen a la discriminación de los genotipos fueron: anchura del fruto, peso de la pulpa, pH, longitud y espesor de semillas, y son las más significativas para la selección de genotipos de S. mombin. Las contribuciones más pequeñas a la diversidad se deben a: la masa del fruto, anchura de semilla, espesor, volumen y longitud del fruto, así como a la masa de las semillas y el contenido de sólidos solubles totales. Ambos métodos de agrupación, UPGMA y Tocher, revelaron que existe divergencia genética entre los genotipos analizados. Los genotipos 37 y 41 son los más divergentes respecto a los otros; por eso son ideales para programas de mejoramiento genético con miras a la domesticación de la especie.