ABSTRACT
The enzymes Acetylcholinesterase (AchE) and Lactatedehydrogenase (LDH) are used as biological markers in the present study. Enzymes are highly sensitive and used to evaluate the biological effects of organophosphate pesticide chlorpyrifos in freshwater mussel Lamellidens marginalis. The test organisms were exposed to sub-lethal concentration (5 ppm) of chlorpyrifos for 30 days and allowed to recover for seven days. A distinct reduction of the enzyme AchE (34 + 3.3 U l-1) was found in the treated hepatopancreas. A significant increase in LDH activity in gill, hepatopancreas and muscle was observed. There was a significant recovery in AchE and LDH in the different tissues, after seven days recovery period.. Hence, the changes in the enzymes are found as the best biomarkering tool to evaluate the effect of organophosphate pesticide chlorpyrifos on the aquatic biota.
ABSTRACT
Rubinstein Taybi syndrome or Broad Thumb and Hallux syndrome is a genetic multisystem disorder characterized by facial dysmorphism, growth retardation and mental deficiency. A ten-year-old boy who had come to the Department of Pedodontics, Ragas Dental College, Chennai, with the chief complaint of unaesthetic appearance with extra teeth revealed delay in the developmental milestones. The patient appeared to be hyperactive and mentally retarded. Extra oral features revealed distinctive facial appearance with a broad fore head, hypertelorism, broad nasal bridge and beaked nose. Intra oral features observed were talons cusps in the upper central and lateral incisors, mesiodens, carious teeth and plaque accumulation. Since the patient was hyper active and mentally retarded, the dental treatment was done under GA. The treatment plan and dental management of this patient are discussed in this case report.
ABSTRACT
Conservative procedures using dentin-bonding agents are one of the important aspects of pediatric dental practice. The objectives of this in vitro study was to comparatively evaluate the tensile-bond strength, fracture mode (under SEM) and microleakage of total etching single bottle system to self-etching adhesive system in primary dentition. The flat buccal/lingual surfaces of 20 teeth were divided into two groups and treated with Single Bond (Group 1) and Adper Prompt (Group 2) to develop a composite resin cone. Then tensile-bond strength was measured using Instron machine. Fracture mode was evaluated in three specimens from each group under SEM. Microleakage of Class V composite restorations (in 20 teeth) with the above-mentioned adhesives was assessed under stereomicroscope after Basic fuschin dye immersion. Results showed no statistically significant difference between two groups. It was concluded that concerning the single step application with similar efficacy, the self-etching adhesive is better for bonding in primary dentition.