ABSTRACT
The specific antibody responses were compared among susceptible (A/Sn), moderately susceptible (Balb/c) and resistant (C57 BL/lOJ) mice infected with Trypanosoma cruzi (Y strain). Sera obtained during the second week of infection recognized a surface trypomastigote antigen of apparent Mr 80 kDa while displaying complex reactivity to surface epimastigote antigens. Complex trypomastigote antigens recognition was detected around the middle of the third week of infection. No major differences were observed along the infection, among the three strains of mice, neither in the patterns of surface antigen recognition by sera, nor in the titres of antibodies against blood trypomastigotes (lytic antibodies), tissue culture trypomastigotes or epimastigotes. On immunoblot analysis, however, IgG of the resistant strain displayed the most complex array of specificities against both trypo and epimastigote antigens, followed by the susceptible strain. IgM antibodies exhibited a more restricted antigen reactivity, in the three mouse strains studied. Balb/c sera (IgG and IgM) showed the least complex patterns of reactivity to antigens in the range of 30 kDa to 80 kDa. The onset of reactivity in the serum to trypomastigote surface antigens was also dependent on the parasite load to which the experimental animal was subjected
Subject(s)
Mice , Animals , Antibodies, Protozoan/immunology , Antigens, Protozoan/immunology , Chagas Disease/immunology , Trypanosoma cruzi/immunology , Antigen-Antibody Reactions , Blotting, Western , Disease Susceptibility , Electrophoresis, Polyacrylamide Gel , Immune Sera , Immunoglobulin Isotypes/analysis , Mice, Inbred A , Mice, Inbred BALB C , Mice, Inbred C57BLSubject(s)
Humans , DNA Probes , Malaria/diagnosis , Recombinant Proteins , RNA Probes , Antigens/urine , DNA, Recombinant , Malaria/epidemiology , Seroepidemiologic StudiesABSTRACT
A method for a rapid and accurate demonstrations of antibodies to Trypanosoma Cruzy by using nitrocellulose paper strips with antigens of the parasite was developed and evaluated. We report herein additional advantages of its use for the detection of specific antibody to Trypanosoma Cruzi in the sera of mice. Increased sensibility without increase in the background was achieved using parasite antigens obtained by mild non-ionic detergent extraction as compared to antigens extracted by alkaline digestion.