ABSTRACT
OBJECTIVE: The purpose of the study was to determine the effects of co-culture with oviductal epithelial cells and Vero cells on mouse embryo. METHOD: For the control group, mouse embryos were cultured alone in Ham's F-10 with 10% FBS. Subcultured oviductal epithelial cell and Vero cell were cocultured in Ham's F-10 with 10% FBS with the mouse embryo and used as the treatment group. Development of mouse embryos were observed. Result: The development rate and hatching rate of embryos that cocultured with oviductal epithelial cell and Vero cell was significantly higher (p<0.05) than control group. When subcultured oviductal epithelial cells were co-cultured with mouse embryo, there was no significant difference in development rate and hatching rate among subculture step. When oviductal epithelial cells that have been frozen-thawed were co-cultured with mouse embryo, there was no significant difference in development rate and hatching rate among subculture step. No statistical significance was seen in the development rate and hatching rate between subcultured oviductal epithelial cells and frozen-thawed oviductal epithelial cells when cocultured with mouse embryo, Vero cells and frozen-thawed when cocultured with mouse embryo, and Vero cells and oviductal epithelial cells when cocultured with mouse embryo. CONCLUSION: Oviductal epithelial cells and Vero cell may have a stimulatory role in early mouse embryonal development compared to control in vitro. As well, there is no significant difference in development rate and hatching rate among subculture step, when early mouse embryo was cocultured with cells that subcultured and frozen-thawed.
Subject(s)
Animals , Humans , Mice , Coculture Techniques , Embryonic Structures , Epithelial Cells , Oviducts , Vero CellsABSTRACT
Imperforate hymen is a rare congenital malformation. Two cases of imperforate hymen regarded to congenital origin, and formed hematocolpometra. In most instances definite finding is noticed after menarche as retention of menstrual flow results in abdominal pain, distension of the lower abdomen and often in acute urinary retention. Two cases of imperforate hymen associated with hematocolpora, hematometra and abdominal distension at age of 14 and 16 were treated satisfactorily by simple crucial incision of hymen, and drained of about 800 cc and 600 cc of tarry blood respectively. We present two cases of imperforate hymen with review of the literatures.
Subject(s)
Female , Abdomen , Abdominal Pain , Hematometra , Hymen , Menarche , Urinary RetentionABSTRACT
Pelvic actinomycosis is a chronic suppurative and granulomatous disease with multiple abscess and draining sinus tracts caused by Actinomyces, a Gram-positive, anaerobic, non-acid-fast bacterium. Pelvic actinomycosis is a rare disease, but it is more common with intrauterine device users than others. The symptoms and signs of pelvic actinomycosis are variable, from asymptomatic to mimicking the symptoms and signs of pelvic mass or acute peritonitis, and sometimes it is difficult to discriminate pelvic malignancy from actinomycosis. Identifying the typical "sulfur granule" with hematoxylin-eosin stain and detecting the actinomycotic colony in Gram stain may suggest the diagnosis. We have experienced a case of pelvic actinomycosis complicated by right tuboovarian abscess in a 39-year-old woman using intrauterine device and report it with brief review of literatures.
Subject(s)
Adult , Female , Humans , Abscess , Actinomyces , Actinomycosis , Diagnosis , Intrauterine Devices , Peritonitis , Rare DiseasesABSTRACT
Pelvic actinomycosis is a chronic suppurative and granulomatous disease with multiple abscess and draining sinus tracts caused by Actinomyces, a Gram-positive, anaerobic, non-acid-fast bacterium. Pelvic actinomycosis is a rare disease, but it is more common with intrauterine device users than others. The symptoms and signs of pelvic actinomycosis are variable, from asymptomatic to mimicking the symptoms and signs of pelvic mass or acute peritonitis, and sometimes it is difficult to discriminate pelvic malignancy from actinomycosis. Identifying the typical "sulfur granule" with hematoxylin-eosin stain and detecting the actinomycotic colony in Gram stain may suggest the diagnosis. We have experienced a case of pelvic actinomycosis complicated by right tuboovarian abscess in a 39-year-old woman using intrauterine device and report it with brief review of literatures.
Subject(s)
Adult , Female , Humans , Abscess , Actinomyces , Actinomycosis , Diagnosis , Intrauterine Devices , Peritonitis , Rare DiseasesABSTRACT
Beckwith-Wiedemann syndrome(BWS) is a group of disorders having in common coexistence of a macrosomia, macroglossia, abdominal wall defects, visceromegaly, hypoglycemia in the neonatal period and embryonal cancers of infancy and early childhood. Beckwith-Wiedemann first reported this distinct clinical entity, and about 200cases have subsequently been reported. Etiology is usually sporadic. BWS is a multigenic disorder with dysregulation of the expression of imprinted genes involved in growth and cell cycle control in the 11p15.5 chromosomal region. Placental endocrine dysfunction leading to increased levels of growth hormone and insulin-like growth factors would causes the visceromegaly. Early visceromegaly may pedispose to omphalocele, malrotation anomalies, and diaphragmatic herniation. Detection and treatment of hypoglycemia in any neonate with features of this syndrome are critical. The frequency of hypoglycemia in this population is between 30 and 50%. The majority of infants with hypoglycemia will be asymptomatic and have resolution of the hypoglycemia within the first 3 days of life. Less than 5% of infoots will have hypoglycemia beyound neonatal period one requive either continous feeding or a partial pancreatectomy. We have experienced and case of Beckwith-Wiedemann syndrome so that we report with brief review of the concerned literature.
Subject(s)
Humans , Infant , Infant, Newborn , Abdominal Wall , Beckwith-Wiedemann Syndrome , Cell Cycle Checkpoints , Growth Hormone , Hernia, Umbilical , Hypoglycemia , Macroglossia , Neoplasms, Germ Cell and Embryonal , Pancreatectomy , SomatomedinsABSTRACT
Twenty-nine patients haboring primary or recurrent malignant gliomas were treated with direct implantation of radioactive sources into the tumor in afterlozded, removable catheters using stereotatic technique between November 1987 and October 1990. Indine-125(I-125) was implanted in 22 patients and high dose rate(HDR) Iridium-192(Ir-192) in 7 patients. All patients had been treated with external radiation and most had been treated with chemotherapeutic agents. one or more sources were placed in each tumor to deliver 5000-8000 cGy to the tumor's periphery for I-125 and 3000 cGy for HDR Ir-192. Eleven of 13 patients with glioblastoma multiforme were alive at 35 weeks whose mean survival was 74 weeks, and remained two were expired at 35 and 78 weeks, respectively. All 5 patients with anaplastic astrocytoma were alive at 39 weeks whose mean survival was 74 weeks. Five of 9 recurrent blioblastoma were alive at 22 weeks whose mean survival was 44 weeks, and remained three were expired at 13, 17 and 22 weeks respectively. All two patients with anaplastic astrocytoma were alive at 22 and 52 weeks, respectively. Four patients with deep seated analphastic astrocytoma(3 in the thalamus and 1 in the third ventricle) were treated successfully by brachytheray without any surgical morbidity and mortality and they were still alive 38, 48, 74 and 123 weeks after brachytherapy. There were few minor postoperative complications including 3 meningitis. 2 leak of cerebrospinal fluid and 3 radiation necrosis without any mortality. The authors conclude that the brachytherapy with temporary implanted I-125 or Ir-192 sources may provide long-term survial in selected patients with primary or recurrent malignant gliomas.