ABSTRACT
<p><b>OBJECTIVE</b>To explore effects of Shenqi preparation,Traditional Chinese Medicine, on anti-fatigue and anti-oxidant functions.</p><p><b>METHODS</b>One hundred and twenty mice were randomly divided into control group and 3 experimental groups. The high, medium and low-dose of Shenqi preparation were given to the 3 experimental groups respectively, while distilled water to the control group for 15 d. The loaded swimming time, the level of lactate, serum urea nitrogen (SUN), muscle and liver glycogen, liver super-oxide dismutase (SOD), the content of malondialdehyde (MDA), glutathione peroxidase(GSH-Px) were assayed.</p><p><b>RESULTS</b>The loaded swimming test showed that the exhausted swimming time of 3 experimental groups [(296.0 +/- 25.3)s, (437.0 ĝ 38.9)s, (595.0 +/- 53.9)s respectively] was longer than that of control group [(231.0 +/- 22.5)s, P < 0.05, P < 0.01]. The liver glycogen content of the high and medium-dose experimental groups were higher than that of control group respectively (P < 0.01). The SUN content of each experimental group was less than that of the control group (P < 0.01, P < 0.05). Moreover,in the medium and high dose experimental groups, less accumulation of lactate was found (P < 0.01, P < 0.05), and the content of liver SOD and GSH-Px was higher (P < 0.01, P < 0.05). The content of liver MDA in high-dose experimental group was less than that of the control group (P < 0.05).</p><p><b>CONCLUSION</b>Shenqi preparation, especially the high and medium-dose experimental groups, is able to improve exercise tolerance and has anti-fatigue and anti-oxidant effects in mice.</p>
Subject(s)
Animals , Male , Mice , Antioxidants , Metabolism , Blood Urea Nitrogen , Disease Models, Animal , Drugs, Chinese Herbal , Pharmacology , Fatigue , Drug Therapy , Metabolism , Glutathione Peroxidase , Metabolism , Glycogen , Metabolism , Lactic Acid , Blood , Liver , Metabolism , Malondialdehyde , Metabolism , Physical Conditioning, Animal , Superoxide Dismutase , MetabolismABSTRACT
Objective: To investigate the therapeutic effect of oat β-glucans on serum lipid levels of rats with experimental hyperlipemia. Methods: Rats were fed on high fat forage for 3 weeks to establish hyperlipemia models, then the model rats were orally given oat β-glucans at doses of 133, 266 and 533 mg/(kg · d) separately. At the same time, there were 3 control groups including chitosan ligosaccharide[200 mg/(kg · d)], normal, and high blood lipid (model rats fed with distilled water). Serum levels of total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were assayed in rats of all groups and the results were compared. Results: β-glucans obviously decreased the levels of TC and LDL-C and increased HDL-C in rat hyperlipemia models. The reduction of serum lipid level in 4-week β-glucans treatment rats was more obvious than that in 3-week β-glucans treatment rats. Besides, β-glucans showed an obvious dosage dependent effect in reducing serum lipid level without adverse effect. Oat β-glucans had a better lipid-reducing effect than ligosaccharide did. Conclusion: Oat β-glucans can obviously decrease serum lipid in rats with hyperlipemia.