ABSTRACT
Objective/background: Collection of one spot and one morning sputum specimen is recom-mended for tuberculosis [TB] drug resistance surveys. This was a retrospective analysis of Mycobacterium tuberculosis cultures isolated from two spot sputum specimens collected from smear positive TB patients in a TB drug resistance survey. It was conducted to under-stand the value of a second specimen
Methods: A TB drug resistance survey was conducted in the state of Tamil Nadu, India, to estimate the prevalence of drug resistance among new sputum smear-positive [NSP] and previously treated [PT] patients diagnosed in Revised National Tuberculosis Control Program microscopy centers. A total of 2425 patients [1524 NSP and 901 PT cases] were enrolled in the study. From these patients, two spot sputum specimens [C and D] were collected within a period of 2 h. No preservative was added to sputum. The samples were transported at ambient conditions without cold storage to the central laboratory for culture of M. tuberculosis. Culture yield from each sample was computed and analyzed. Results: The proportion of cultures retrieved from C and D specimens among NSP cases [89.3% and 89.7%] and PT cases [90.8% and 90.3%] were similar. The culture grades of C and D samples were comparable [chi-square test, 3560.135; p < .001] and the agreement was moderate [kappa test, 0.454]
Conclusion: The findings of the study reveal the adequacy of single spot sputum specimen from smear positive pulmonary TB patients for bacteriological examination in a quality-assured TB laboratory to determine precisely the level of drug resistance in a province of India