Antrodia cinnamomea exerts an anti-hepatoma effect by targeting PI3K/AKT-mediated cell cycle progression in vitro and in vivo

Antrodia cinnamomea is extensively used as a traditional medicine to prevention and treatment of liver cancer. However, its comprehensive chemical fingerprint is uncertain, and the mechanisms, especially the potential therapeutic target for anti-hepatocellular carcinoma (HCC) are still unclear. Using UPLC‒Q-TOF/MS, 139 chemical components were identified in A. cinnamomea dropping pills (ACDPs). Based on these chemical components, network pharmacology demonstrated that the targets of active components were significantly enriched in the pathways in cancer, which were closely related with cell proliferation regulation. Next, HCC data was downloaded from Gene Expression Omnibus database (GEO). The Cancer Genome Atlas (TCGA) and DisGeNET were analyzed by bioinformatics, and 79 biomarkers were obtained. Furtherly, nine targets of ACDP active components were revealed, and they were significantly enriched in PI3K/AKT and cell cycle signaling pathways. The affinity between these targets and their corresponding active ingredients was predicted by molecular docking. Finally, in vivo and in vitro experiments showed that ACDPs could reduce the activity of PI3K/AKT signaling pathway and downregulate the expression of cell cycle-related proteins, contributing to the decreased growth of liver cancer. Altogether, PI3K/AKT-cell cycle appears as the significant central node in anti-liver cancer of A. Cinnamomea.

Study on the influencing factors and mechanism of medical staff′s job satisfaction based on grounded theory / 中华医院管理杂志

Objective:To explore the influencing factors and mechanism of job satisfaction of medical staff based on grounded theory.Methods:A total of 33 literatures published in CNKI from January 1, 2015 to December 31, 2019 were retrieved with the theme of " job satisfaction of medical staff" . By using NVivo 11.0 software, the 33 literatures were qualitatively analyzed, and the main categories influencing the job satisfaction of medical staff were obtained, and the action mechanism model was integrated on this basis.Results:The influencing factors of medical staff′s job satisfaction were summarized into 6 first-class nodes: work ecology, hospital management, basic health care, occupational cognition, individual characteristics and external environment. Among them, work ecology was the direct influence factor; hospital management was the key influencing factor, which played an important role in the work ecology; basic health care was the safeguard factor, which was affected by the work ecology; occupation cognition was the internal driving factor, individual characteristic was the internal uncontrollable factor, external environment was the external uncontrollable factor.Conclusions:In order to improve the job satisfaction of medical staff, we should optimize the work ecology, promote the innovation of hospital management, ensure the health and safety, and pay attention to the internalization of professional values.