ABSTRACT
Objective To evaluate the effect of Ganoderma lucidum on drug resistance of the cisplantin-resistant ovarian cancer cell line(A2780CP).Methods The A2780CP cells were randomly divided into control group and test group.The cells in control group and test group were incubated in culture media alone and in culture media containing Ganoderma lucidum 0.5 mg/ml for 48 h respectively.The resistance index(RI) of A2780CP was determined by WST-1 assay.The expression of Akt,Bcl-2 and p53 protein was measured by Western blot.Results The RI was significantly deeressed,the expression of Akt and Bcl-2 protein was down-regulated,while p53 protein expression was up-regulated in test group as compared with control group(P<0.05).Conclusion Ganoderma lueidum can reduce the resistance of A2780 CP cells to cisplantin by down-regulating the expression of Akt and Bcl-2 and up-regulating p53 expression in A2780CP cells.
ABSTRACT
Objective To discuss nursing measures for drainage through central venous catheter for treatment of patients with end-stage liver cirrhosis and obstinate ascite of liver cancer. Methods A total of 76 patients with end-stage liver cirrhosis and obstinate ascite of liver cancer were divided into the ob-servation group(40 cases)and the control group(36 cases).The observation group was given drainge of ob-stinate ascite through central venous catheter as well as nursing observation and records based upon routine treatment,which was adopted by the control group.The treatment effect was compared between the two groups. Results The effective rate of treatment in the observation group was higher than that of the con-trol group while the incidence rate.of complication was lower,P < 0.05. Conclusions Utilization of drainge of obstinate ascite through central venous catheter has advantages of little trauma and pain,preven-tion of infection by repeated operation,desirable effect and low medical cost. Circumspect preoperative preparation and postoperative nursing ensured the success of treatment.