Effect of pinellia extract on HeLa cell line in vitro and associated mechanism / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To investigate the effect of Pinellia extract (PE) on HeLa cell line and to study its associated mechanisms, in order to provide theoretical foundations for its applying in clinical prevention and treatment of cervical disease.</p><p><b>METHODS</b>HeLa cell line was incubated in media containing different concentrations of PE. Growth of cells was observed and photo-generated with inverted phase microscope; cell activity was detected by MTT assay; cell apoptosis detected by flow cytometry, protein expression of proliferating cell nuclear antigen proliferating cell nuclear antigen (PCNA) was detected by immuno-cytochemistry, and protein expression of Bcl-2 was determined by immunofluorescence.</p><p><b>RESULTS</b>PE showed obvious inhibition on the proliferation of HeLa cells, cell apoptosis appeared after PE treatment in a time and dose dependent manner; PCNA and Bcl-2 protein expressions reduced significantly after being effected by PE for 24 h.</p><p><b>CONCLUSIONS</b>PE can obviously inhibit the growth and induce the apoptosis of HeLa cells, and its mechanism is possibly realized through down-regulating the expressions of PCNA and Bcl-2. The study set a primary experimental base for further studying the action mechanism of PE and developing new anti-tumor agents.</p>

Prelimary exploration on Chinese medicine syndrome type distribution in patients with polycystic ovary syndrome / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To explore the Chinese medicine syndrome type distribution in patients with polycystic ovary syndrome (PCOS) and its relationship with sexual hormones.</p><p><b>METHODS</b>Chinese medicine syndrome types of 212 PCOS patients were differentiated and sorted by adopting fuzzy mean C clustering method, and their relationship with the indices of sexual hormones detected on the 3rd to 5th day of menstrual cycle was analyzed, with the values got from 20 healthy women for controls.</p><p><b>RESULTS</b>Intermingling syndromes were commonly seen in PCOS patients. Shen-deficiency syndrome (presented in 64 patients) and Gan-qi stagnancy syndrome (61 patients) were the dominance, accounting for 30.2% and 28.8% respectively, significantly higher than that of other syndromes (P < 0.05), which were Pi-deficiency syndrome (41 patients, 19.3%), phlegm-dampness syndrome (33 patients, 15.6%) and blood stasis syndrome (13 patients, 6.1%). Levels of estradiol (E2), testosterone (T), luteinzing hormone (LH), dehydroiso-androsterone (DHEA-S) and prolactin (PRL) were higher, while the level of sexual hormone binding protein (SHBG) was lower in PCOS patients than those in control, follicular stimulating hormone (FSH) level in patients of Shen-deficiency syndrome and phlegm-dampness syndrome was high than that in control (P < 0.05 and P < 0.01). However, no significant differences were found in comparing the various sexual endocrinal indices between patients with different syndrome types (P > 0.05). Besides, the level of PRL was positively correlated with LH and E2 levels in patients.</p><p><b>CONCLUSION</b>Chinese medicine syndromes presented in patients with PCOS are mostly intermingling, Shen-deficiency and Gan-stagnancy are the basic syndromes, and there is some correlation between syndrome type and sexual hormone levels.</p>

Effect of Chinese herbal treatment on the efficiency of blocking CD antigen and on prolactin and progesterone in women with recurrent spontaneous abortion / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To explore the effect of Chinese herbal medicine Baotai Granule (BTG, a self-made preparation) on CD antigen blocking efficiency, prolactin (PRL) and progesterone (P) in patients with recurrent spontaneous abortion (RSA).</p><p><b>METHODS</b>Thirty-four women suffered from RSA were treated with BTG, twice every day, 1 package (10 g) in each time by orally intake. Changes of the efficiency of serum blocking antibody in them to the CD antigen in their husband's peripheral T-lymphocytes before and after treatment were observed. And the changes of blood levels of PRL and P were also monitored.</p><p><b>RESULTS</b>Fetus had successfully protected in 30 women (88.2%), in them, the efficiency of blocking to CD3, CD4 and CD8 after treatment were all higher than that before treatment, and levels of PRL and P in peripheral blood increased along with the increase of gestational age, while no obvious change was found in those who failed to complete pregnancy.</p><p><b>CONCLUSION</b>Chinese herbal medicine could protect the fetus by regulating the response between endocrine and immunity network during pregnancy.</p>

Effect of bushen yiqi recipe on bioactivity behavior of human cytotrophoblast of early pregnancy / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To investigate the effect of Chinese drugs for tonifying Shen on the bioactivity, cell proliferation, invasion and differentiation of human cytotrophoblast of early pregnancy.</p><p><b>METHODS</b>The human early pregnant cytotrophoblast was cultured in vitro, and treated with saline (as blank control) and drug containing serum in different concentrations (5%, 10% and 20%) respectively. The changes of morphology, proliferation and invasive capacity of cells were examined by scanning electron microscopy, MTT method, flow cytometry, Transwell invasive assay at 24 hrs, 48 hrs and 72 hrs after treatment.</p><p><b>RESULTS</b>After cells being cultured with drug containing serum, the cytotrophoblast became abundant in microvilli, with more and prolonged pseudopodia. The cell absorbency in 490 nm wave length increased significantly (P < 0.01), cells of sub-G1 and G2/M phase obviously decreased and that of S phase increased (P < 0.01), and the cells penetrated through PET membrane in each visual field significantly increased (P < 0.01).</p><p><b>CONCLUSION</b>Chinese herbs for tonifying Shen could promote the proliferation and invasive capacity of cytotrophoblast and might influence its differentiation.</p>