ABSTRACT
Objective: To investigate the relation between ser um hyaluronic acid (HA) concentration and cryopreservation-reperfusion injury. Methods: The animals were randomly assigned to 3 groups: (1 ) group A: the control; (2) group B: liver allografts were stored in lactated R inger's solution (0℃) for 2 h before implantation; (3) group C:liver allografts were stored in lactated Ringer's solution (0℃) for 4 h before implantation. Th e serum sample and liver specimen were taken up at 2 h and 4 h after transplanta tion to detect the concentration of HA, AST and LDH, and to get pathologic obser vation. Results: Serum HA increased earlier and decreased more s hortly than AST and LDH after transplantation in group A. Serum HA increased sig nificantly in group B and C, much higher than that in group A(P<0.01). The i njury of vascular endothelium and the disorder of hepatic sinuses and hepatic lo b ules were observed in group B and C. In the specimen of 4 h in group C, evident infiltration of inflammatory cell was present. Conclusion: Cryopreservation leads to injury of endothelial cell and reperfusion aggravat es this injury. The serum HA concentration indicates the degree of cold ischemia -reperfusion injury.
ABSTRACT
A cuff technique was applied to anastomose pulmonary vein,pulmonary arery andbronchus in rat receiving lung transplantation.In 6 consecutive cases,the average graft ischemictime was 17±3 rain,and the time for operation was 61±4 min.The time for ischemia was 1essthan one third of previous reports and the time for operation one forth of previous reports.Theoperation successful rate was 75%,and the survival rate was 100%on the postoperative day 7.After contralateral pneumonectomy,the blood gas analysis was normal,showing excellent graftfunction of the oxygenaration of the animals.