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OBJECTIVE To analyze the pathogens of severe pneumonia induced by different underlying diseases in(hospital) and to evaluate the difference of the pathogens to antimicrobial susceptibility test.METHODS The(bacteria) through sputum culture with VITEK-AMS,and the antimicrobial susceptibility against(bacteria) by K-B method were tested in hospital from 2002 to 2005.RESULTS There were 106 patients with 173 strains of isolated(pathogenic) bacteria,including Klebsiella pneumoniae(KPA) 20.9%,and Escherichia coli(ECO)(16.3%) from the aspiration severe pneumonia;Pseudomonas alcaligenes(PAL) 18.9%,P.aeruginosa(PAL)(16.2%) and Staphylococcus aureus(SAU) 16.2% from the obstructive severe pneumonia;KPN 22.6%,SAU(18.9%),and PAE 17.0% from the COPD complicated with severe pneumonia and PAE 25.0% and SAU 20% from the(hospital-)acquired pneumonia(HAP).CONCLUSIONS The constructed ratio of pathogens is different between(severe) pneumonia infected by different underlying diseases and community-acquired pneumonia without any(underlying) disease.The resistant pathogens are increasing significantly in cases with HAP.
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Objective To detect the expression of inositol 1,4,5-triphosphate receptor (IP 3R) subtypes in normal rat airway smooth muscle cells (ASMCs) and changes during chronic asthma formation. Methods ASMCs were cultured by collagen enzyme digestion method. The expressions of subtypes of IP 3R were detected by RT-PCR and the purified PCR products were linked with pGEM-T vector for DNA sequencing. Chronic asthma model was established with egg albumin. The changes of IP 3Rs were detected by RT-PCR method. Results All subtypes of IP 3R were expressed in airway smooth muscle cells of normal rats. The expression of IP 3R1 in asthma groups increased obviously as compared with that in the control group (P
ABSTRACT
The study on the functions of the diaphragm has been carried on for 5 years.It consisted of the studies on the relationship of the tension and endurance of inspiratory muscles with pulmonary functions,the changes of diaphragmatic electromyogram in maximal ventilation volume test in normal subjects and patients with chronic obstructive pulmonary disease(COPD),the functional changes of the diaphragm in dogs with RDS and the therapeutic effects of aminophylline.the changes of diaphragmatic electromyogram in dogs with hypoxemia and hy-percapnia,determination of transdiaphragmatic pressure,the relationship of the stimulation frequency on the phrenic nerve with diaphragmtic fatigue,etc.It was found that there existed di-aphragmtic fatigue in dogs with RDS,in dogs with hypoxemia and hypercapnia,and in patients with COPD,and aminophylline,digoxin,salbutomal ?-receptor excitory agents can minimize or prevent diaphragmatic fatigue.