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OBJECTIVE To investigate the ?lactamase genes in Escherichia coli and their resistance.METHODS Twenty strains of E.coli producing extended spectrum ?-lactamases(ESBLs) were isolated from hospitalized patients.?-Lactamase drug-resistant genes were detected by PCR.RESULTS There were higher positive rates of ?-lactamase genes of TEM,SHV,LEN,CTX-M-1,CTX-M-9,VEB,GES,PER(75.0%,20.0%,95.0%,15.0%,70.0%,5.0%,85.0% and 70.0%,respectively). This study showed that there were high positive rate of drug resistance.CONCLUSIONS There are very high positive percentages of TEM,LEN,CTX-M-9,GES,and PER genes in ESBLs producing E.coli isolated clinically,and showing highly drug-resistant E.coli of producing ESBLs can be transmitted in hospital and induce fulminant prevalence.
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OBJECTIVE To detect the disinfectants-resistant gene of qacE-sul1 in nosocomial infected patients.METHODS The disinfectants-resistant gene of qacE-sul1 was detected by polymerase chain reaction(PCR).RESULTS The positive rate of gene of qacE-sul1 in 222 strains was 54.5%.The positive rate of gene qacE-sul1 in Klebsiella pneumoniae,Escherichia coli,Staphylococcus aureus,Proteus,Pseudomonas aeruginosa,Enterococcus,and Acinetobacter baumannii were 47.6%,58.3%,57.6%,54.2%,53.6%,53.3%,and 55.0%,respectively.CONCLUSIONS There are higher positive percentages of gene qacE-sul1 in nosocomial infected patients which possibly causes nosocomial infection and should be attention to.
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OBJECTIVE To investigate the genetic polymorphism of imipenem-resistant Pseudomonas aeruginosa by randomly amplified polymorphic DNA(RAPD). METHODS The genes of imipenem-resistant P.aeruginosa were amplified by RAPD assay in 8 clinical isolates and PCR products were analyzed by agarose gel electrophoresis and cluster analysis. RESULTS The phenotypes of drug resistance were the same,but most of the genotypes were different,even though some of them could be equal. CONCLUSIONS RAPD assay reveals the special genotypes of imipenem-resistant P.aeruginosa and makes the relation between the phenotype of drug resistance with genotype of drug resistance definite,which provides molecular epidemiological evidence for strategies to control resistant imipenem P.aeruginosa infections.
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OBJECTIVE To investigate the drug-resistance and the existence of genes in 16S rRNA methylases and aminoglycoside modifying enzymes in strains continuously isolated from Pseudomonas aeruginosa(PAE) in two hospitals of Jiangsu and Zhejiang Provinces.METHODS The drug-resistance of the strains continuously isolated from PAE was detected with K-B test,five kinds of genes in 16S rRNA methylases(rmtA,rmtB,rmtC,rmtD and armA) and six kinds of aminoglycoside modifying enzymes [aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰb,aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰ] were detected by PCR.RESULTS The strains were just sensitive to cefoperazone/sulbactam,imipenem,meropenem and amikacin(hospital A:74.2%,80.0%,82.9% and 68.5%;hospital B:90.0%,50.0%,50.0% and 95.0%,respectively).There was a high rate in the drug-resistance to ?-lactamase medicines,ciprofloxacin and sulfamethoxazole co.Genes in 16S rRNA methylases were not detected from PAE strains in the two hospitals.CONCLUSIONS The rates of genes in aminoglycoside modifying enzymes detected from strains in continuously isolated from PAE are different in different hospitals.
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OBJECTIVE To investigate the plasmid AmpC genes,?-lactamases genes and intⅠgenes in Escherichia coli.METHODS M-H test was performed to detect the antimicrobial susceptibility of 40 strains of E.coli isolated from the clinic.?-Lactamases genes,plasmid AmpC genes and intⅠgenes were detected by PCR methods.RESULTS Forty strains of E.coli were multiple-drug-resistant.The positive rate of genes of TEM,SHV,LEN,CTX-M-1,CTX-M-9,VEB,GES and PER was 55.0%,7.5%,55.0%,12.5%,45.0%,5.0%,45.0% and 35.0%,respectively.The positive rate of plasmid AmpC genes of ACT-1 and DHA was 57.5% and 40.0%,respectively.The positive rate of intⅠgenes was 47.5%.CONCLUSIONS E.coli isolated from the clinic is multiple-drug-resistant and wityh high positive rate of the plasmid AmpC genes,?-lactamases genes and int Ⅰ genes.
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OBJECTIVE To investigate the antibiotic resistance and the genetical marks in Pseudomonas aeruginosa(PAE) strains in two hospitals of Jiangsu and Zhejiang.METHODS The drug-resistance in PAE strains was detected with K-B test,four kinds of genetical marks of integron and transposon(qacE△1-sul1,mer,tnpA and tnpU) were detected by PCR.RESULTS The strains were only sensitive to cefoperazone/sulbactam,imipenem,meropenem and amikacin.There was a high resistance rate to ?-lactamases,ciprofloxacin,and sulfamethoxazole.Genes of qacE△1-sul1(48.6%,45.0%) and merA(11.4%,5.0%) were detected from PAE strains in the two hospitals.There were no tnpA and tnpU too.CONCLUSIONS Multiple-drug-resistance in Pseudomonas aeruginosa(PAE) strains is caused mainly by qacE△1-sul1 and merA.