ABSTRACT
Natural products especially from plants have been used for the treatment of various diseases. Momordica charantia or bitter melon, grows in the tropical area is popularly consumed as vegetables and has high medicinal values .It is one of the most promising alternative medicines used as anti-HIV, anti-ulcer, antiinflammatory, antileukemic, anti-microbial, anti-diabetic, and anti-tumor. Proteins like momordin, alpha- and betamomorcharin and cucurbitacin B of Momordica charantia were also tested for possible anticancerous effects .Chemical analogs of these proteins have been developed, patented, and named MAP30.The MAP30gene was amplified from Momordica charantia leaves by Polymerase Chain Reaction .The resultant product was amplified approximately 861 bp.
ABSTRACT
Tobacco streak virus (TSV) is an important emerging virus belongs to the genus Ilarvirus and family Bromovidae. The Onion crop is infected by Tobacco streak ilar virus and it is major problem in different places of Andhra Pradesh in South India and also transmitted by thrips vector. TSV suspecting onion samples were identified by direct antigen coating enzyme linked immunosorbent assay using TSV polyclonal antiserum. The Replicase gene from each isolate was amplified using TSV replicase gene specific primers by using the RT-PCR. The ~530 bp product was amplified, cloned, sequenced and determined its length as 534 nucleotides and codes for 178 amino acids. The partial sequence of TSV-Rep shared identity of 87.6 -99.8% at nucleotide levels and 67.8- 99.4% at amino acid levels respectively with other reported TSV isolates. The phylogenetic tree relationship based on the nucleotide sequence of present study isolate (AP-Onion-Chittoor) from different geographical regions was also analyzed in this study.