ABSTRACT
We used a novel DNA fingerprinting probe O-chi-1 (ref. 1) to detect differences in the hybridization pattern of brain tumor DNA and paired normal tissue of a given individual. Representatives of meningiomas (two), glioblastoma multeforme (three) and astrocytoma (one) were studied. Alterations, which included amplification as well as the loss of a normal band in tumor DNA, were observed in four of the six tumours. While the increased intensity of a band can be taken to imply increased copy number, the disappearance of bands could either be due to loss of DNA sequence or rearrangement resulting in different sized bands.
Subject(s)
Astrocytoma/genetics , Blotting, Southern , Brain Neoplasms/genetics , DNA Fingerprinting/methods , DNA Probes , DNA, Neoplasm/chemistry , Deoxyribonucleases, Type II Site-Specific/metabolism , Electrophoresis, Agar Gel , Glioblastoma/genetics , Humans , Meningioma/geneticsABSTRACT
Various storage treatments on human blood samples have been described with respect to DNA yield, quality and fingerprinting. Blood samples were stored at room temperature, 4 degrees C, -20 degrees C and -70 degrees C for different duration varying from 1, 2, 4 and 8 weeks with or without anticoagulant/preservative (EDTA or Heparin). DNA was isolated from these stored samples, quantitated by spectrophotometry and subjected to DNA fingerprinting using a human alphoid satellite DNA sequence (TRF 5.6) and a 33 mer oligonucleotide (O-chi-1) as probes. The polymorphic DNA bands were apparent between 2 to 9 kb size range and the fingerprints were individual-specific. Our results suggest that higher amount of genomic DNA can be recovered from blood samples stored at temperatures 4 degrees C or below in the presence of EDTA or heparin.
Subject(s)
Blood Preservation/adverse effects , DNA/analysis , DNA Fingerprinting , Forensic Medicine , HumansABSTRACT
Eight stocks of Aedes aegypti-4 geographic stocks and 4 selected stocks-were tested for their susceptibility to infection with Dirofilaria immitis and D. repens. Four of the stocks were found to be susceptible and the other 4 were refractory of infection with D. immitis. All the stocks of Ae. aegypti that were susceptible to D. immitis were also susceptible to D. repens but the stocks that were refractory to D. immitis were all partially susceptible to D. repens.
Subject(s)
Aedes/parasitology , Animals , Dirofilaria immitis/growth & development , Filarioidea/growth & development , Host-Parasite InteractionsABSTRACT
The susceptibility of Dirofilaria repens infection in Aedes aegypti adults reared in hay-infusion and tap-water during their larval stages was tested. Ae. aegypti reared in hay-infusion ingested a bigger amount of blood than those reared in tap-water but harboured fewer D. repens larvae (developing and arrested). The difference in the susceptibility of mosquitoes from the two treatments was correlated with the difference in the robustness of the mosquitoes.