ABSTRACT
Aims: This paper describes the In vitro study of protein binding by sildenafil citrate (SC) in presence of bisoprololfumarate (BF) and metformin hydrochloride (MH). Study Design: Study was designed to assess In vitro of quenching of bovine serum albumin (BSA) by sildenafil citrate (SC) in presence of bisoprolol fumarate (BF) and metformin hydrochloride (MH) by fluorescence spectrophotometry. Place and Duration of Study: Drug Analysis and Research Laboratory, Centre for Advanced Research in Sciences, University of Dhaka, Dhaka-1000, Bangladesh between December 2013 and March 2014. Methodology: In the present work, the In vitro study of quenching of BSA by SC in presence of BFand MH have been studied by fluorescence emission spectroscopy under different conditions. At first, the BSA solution (20 μM) was prepared in phosphate buffer (pH =7.4) in eight test tubes and different amounts of sildenafil citrate was added to each BSA solution to obtain the final concentrations as 0, 20, 40, 80, 120, 160,240 and 320 × 10-6 molL-1, respectively. Then the fluorescence emission spectra of BSA-SC system were recorded for eight test tubes at two excitation wavelengths of BSA (λExmax= 280 nm and λExmax=293 nm) at 298 K and 308 K. Similarly, the fluorescence emission spectra of BSA-(SC+BF), BSA-(SC+MH) and BSA-(SC+BF+MH) systems were recorded at 280 nm and293 nm at 298 K and 308 K. Quenching constants were determined using the Stern-Volmer equation to provide a measure of the strength of quenching of BSA by SC in presence of BF and MH in all the systems. Results: The quenching of BSA by SC was increased in presence of BF and MH but remained close in presence of both BF and MH. Quenching constants were larger for the BSA-(SC+BF) system and ranked in the order as BSA-(SC+BF)>BSA-(SC+MH)>BSA-(SC+BF+MH)≈ BSA-SC at 280 nm at two different temperatures, respectively. But quenching at the excitation wavelength of 293 nm was ranked in order as BSA-(SC+BF) >BSA-(SC+MH) >BSA-(SC+BF+MH)>BSA-SC at 298 K and 308 K, respectively. Conclusion: It was found that BSA quenched by SC in presence of BF and MH, which indicated that the effectiveness of SC might be predominately influenced by these drugs.
ABSTRACT
Aims: The present study was designed to evaluate the anti-diabetic as well as prophylactic activity of Jambadyarista and Bohumutrantak Ras in alloxan-induced diabetic rats. Study Design: Study the prophylactic and antiglycemic effects against diabetes of two Ayurvedic drugs ‘Jambadyarista’ and ‘Bohumutrantak Ras’ in normal as well as alloxaninduced diabetic rats using in vivo models. Place and Duration of Study: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh between December 2013 and March 2014. Methodology: To investigate the activity, 70 Long Evans rats divided into seven (A-G) groups were included in this study. Diabetes was induced by single intraperitoneal injection of alloxan (150mg/kg body weight) while the Ayurvedic drugs Jambadyarista and Bohumutrantak Ras were given orally at a dose of 200mg/kg of body weight. Group A was control group. Groups B and E were allowed to fast for 12 hours. Diabetes was induced by intraperitoneal injection of freshly prepared solution of alloxan (150mg/kg) in normal saline after base line glucose level determination. The alloxan-treated rats were allowed to food over night to overcome drug induced hyperglycemia. After 48 hours, blood glucose level was measured with an Accu-Chek glucometer using blood sample from the tail vein of each rat. Diabetes was established in animals when blood glucose level was raised to 11.1-32.6mmol/L. After the establishment of diabetes, the experiments were carried out. Groups D and G were allowed to induce diabetes by single intraperitoneal injection of alloxan (75mg/kg body weight) in normal saline every day for 5 days. Results: In case of alloxan-induced diabetic rats, Jambadyarista and Bohumutrantak Ras showed prophylactic activity against diabetes as well as also reduced blood glucose level. By statistical analysis of results, it was found that Jambadyarista and Bohumutrantak Ras have prophylactic activity against diabetes in normal and alloxan- induced diabetic rats. Conclusion: It can be inferred that the Ayurvedic drug “Jambadyarista and Bohumutrantak Ras” significantly possess prophylactic activity against diabetes. They also showed antidiabetic effect in alloxan-induced diabetic rats. Further comprehensive cellular and molecular investigations are required to characterize the exact mechanism responsible for its prophylactic and antidiabetic effects.