ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of three major ginsenosides from mountain ginseng as anticancer substance and explore the underlying mechanism involved in lung cancer.</p><p><b>METHODS</b>The inhibitory proliferation of lung cancer by major five ginsenosides (Rb1, Rb2, Rg1, Rc, and Re) was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Calculated 50% inhibition (IC50) values of five ginsenosides were determined and compared each other. Apoptosis by the treatment of single ginsenoside was performed by fluorescence-assisted cytometric spectroscopy. The alterations of apoptosis-related proteins were evaluated by Western blot analysis.</p><p><b>RESULTS</b>The abundance of ginsenosides in butanol extract of mountain ginseng (BX-MG) was revealed in the order of Rb1, Rg1, Re, Rc and Rb2. Among them, Rb1 was the most effective to lung cancer cell, followed by Rb2 and Rg1 on the basis of relative IC50 values of IMR90 versus A549 cell. The alterations of apoptotic proteins were confirmed in lung cancer A549 cells according to the administration of Rb1, Rb2 and Rg1. The expression levels of caspase-3 and caspase-8 were increased upon the treatment of three ginsenosides, however, the levels of caspase-9 and anti-apoptotic protein Bax were not changed.</p><p><b>CONCLUSION</b>Major ginsenosides such as Rb1, Rb2 and Rg1 comprising BX-MG induced apoptosis in lung cancer cells via extrinsic apoptotic pathway rather than intrinsic mitochondrial pathway.</p>
Subject(s)
Humans , A549 Cells , Apoptosis , Blotting, Western , Butanols , Cell Proliferation , Cell Shape , Cell Survival , Flow Cytometry , Ginsenosides , Chemistry , Pharmacology , Therapeutic Uses , Inhibitory Concentration 50 , Lung Neoplasms , Drug Therapy , Pathology , Panax , Chemistry , Plant Extracts , Pharmacology , Therapeutic Uses , Staining and LabelingABSTRACT
Sodium hypochlorite (NaOCl) is commonly used as a disinfectant or bleaching agent. The ingestion of household bleach is often benign, with minimal irritating effect on the mucosa. Occasionally, however, it can be life-threatening. Here, we report an unusual case of acute poisoning involving household bleach with a near-fatal outcome that was treated with intense hemodialysis. A 42-year-old woman presented to the emergency room after ingesting 1 liter of 5% household bleach. Ten hours later, her metabolic acidosis, hypernatremia, hyperchloremia, and renal failure deteriorated gradually, despite aggressive medical treatment. Rapid, effective correction of the metabolic acidosis and electrolytes imbalance was needed and hemodialysis was performed immediately. After 3 days of dialysis, the laboratory imbalance was completely corrected.
Subject(s)
Adult , Female , Humans , Acidosis , Dialysis , Eating , Electrolytes , Emergencies , Family Characteristics , Hypernatremia , Mucous Membrane , Renal Dialysis , Renal Insufficiency , Sodium , Sodium HypochloriteABSTRACT
Rhabdomyolysis is a serious and potentiallylethal disease that can develop from a variety of traumatic and nontraumatic conditions. In this report, the authors describe a case of rhabdomyolysis that occurredafter a body-building tournament. A 32-year-old body-builder was admitted due to quadriplegia and muscle pain. The patient had a serum potassium level of 1.8 mmol/L, creatinine phosphokinase level of 5,414 IU/L and urine myoglobin of 128.1 ng/ml. He had taken anabolic androgenic steroids for 6 months and overate himself with carbohydrate food after the tournament. Possible causes for the rhabdomyolysis were hypokalemia, exercise, and anabolic androgenic steroids, etc. His condition was fully recovered without complications after potassium replacement and general supportive care. Body- builders may be exposed to rhabdomyolysis risk factors such as diet control, weight reduction, and taking steroids. Therefore, special attention and education on rhabdomyolysis should be provided to body-builders.