Mineral, Nutritional, and Phytochemical Profile, Total Phenolic Content, and Radical Scavenging Activity of Philippine Bamboo "Bolo" Gigantochloa levis (Blanco) Merr. Leaves

The study is a pioneering effort to determine the mineral, nutritional, and phytochemical composition and phenolic content and to determine the free radical scavenging activity of Gigantochloa levis (Blanco) Merr, a native bamboo species (locally known as "bolo") in the Philippines. Proximate analysis showed that air-dried G. levis leaves contain 15.8% ash, 22.6% crude protein, 1.2% crude fat, 29.3% crude fiber, and 19.7% total sugar. Phytochemical tests indicated the presence of diterpenes, triterpenes, saponins, phenols, tannins, and flavonoids in both the ethanolic and aqueous leaf extracts, while phytosterols were only detected in the ethanolic extract. Folin-Ciocalteu assay determined the total phenolic content in gallic acid equivalents (GAE) to be 85.86 ± 3.71 and 32.32 ± 1.01 mg GAE/100 g dried sample for the ethanolic and aqueous extracts, respectively. The total phenolic content in quercetin equivalents (QE) was 74.44 ± 3.11 and 29.43 ± 0.85 mg QE/100g dried sample for the ethanolic and aqueous extracts, respectively. The radical scavenging activity of the different solvent fractions containing varying concentrations of the extract was determined using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ethyl acetate and 1-butanol fractions were found to have the highest radical scavenging activity. Mineral analysis via Energy Dispersive X-Ray Spectrometry (EDS) of the ash of G. levis leaves showed that Si is the major component, followed by K and Mg. These results point to the potential of G. levis leaves as a source of minerals and bioactive compounds with medicinal value.

Anti-hyperglycemic, anti-hypertriglyceridemic and stimulatory effect on glucose transporter 4 mRNA appearance of hydrolysable tannins(Rosanin) of the rosa rugosa root in the streptozotocin-injected diabetic rats / 대한내과학회지

BACKGROUND: The root of the plant Rosa rugosa has been reported to have an anti- hyperglycemic and anti-hyperlipidemic effect in experimental animals. But its definite anti-hyperglycemic and anti-hyperlipidemic substance has not yet been identified. In this study, we found the hydrolysable tannins of the hot water extract of the Rosa rugosa root had those effects, and named it as Rosanin. And its mode of actions were evaluated. METHODS: Three groups of streptozotocin-injected rats and two groups of buffer-injected control rats were given 10 mg/Kg/ml of Rosanin, or glibenclamide, or water by nasogastric tubes for three weeks. Blood sugar, insulin, triglyceride and GLUT4 mRNA levels were checked in the fasting state. Liver, kidney and pancreatic specimens were observed by light microscopy. RESULTS: All the water-treated, glibenclamide-treated and Rosanin-treated diabetic rats had significantly higher blood sugar levels compared to that of normal control rats (p4 gulcoside bonds and phenolic acids, such as ellagic and gallic acid1).