ABSTRACT
A simple, specific and accurate reverse phase liquid chromatographic method has been developed for the simultaneous determination of naproxen and ranitidine HCl. Both the drugs are official with British Pharmacopoeia 2007, but do not involve simultaneous determination of naproxen and ranitidine HCl. The separation was carried out using 4.6x250 mm Symmetry Shield TM RP 18 with a particle diameter of 5 micro m and mobile phase containing 0.1M orthophosphoric acid: methanol [35:65, pH 3.1] in isocratic mode. The flow rate was 1.00 ml/min and effluent was monitored at 240 nm. The retention times [average] of ranitidine HCl and naproxen were 2.36 min and 12.39 min, respectively. The linearity for naproxen and ranitidine HCl was in the range of 5-35 micro g/ ml and 1.5-12 microg/ ml, respectively. The potencies of naproxen and ranitidine HCl were found 99.40% and 99.48%, respectively. The proposed method was validated and successfully applied to the estimation of naproxen and ranitidine HCl in newly formulated combined tablet and in plasma