ABSTRACT
Objective To investigate the changes of C-reactive protein (CRP),fibrinogen (FIB) and erythrocyte sedimentation rate (ESR) in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS),and their relationship with sleep respiratory parameters.Methods From June 2015 to August 2018,70 OSAHS patients [observation group,apnea hypopnea index (AHI) ≥ 5/h] and 20 persons in the snoring control group (control group,AHI ＜ 5/h) who received the monitor of polysomnography in the Pingxiang People's Hospital were enrolled.The levels of CRP,FIB and ESR were measured and compared between the two groups.The correlations were analyzed between the levels of inflammatory markers and sleep breathing parameters.Results The two groups had no statistically significant differences in age,gender and BMI(all P ＞ 0.05).The CRP,FIB and ESR of the observation group were (4.5 ± 3.0) mg/L,(3.3 ± 0.9) g/L,(11.4 ± 5.4) mm/h,respectively,which were significantly higher than those of the control group [(2.0 ± 1.5) mg/L,(2.9 ± 0.5) g/L,(7.6 ± 2.6) mm/h] (t =5.12,2.68,4.36,all P ＜ 0.05).The level of CRP was positively correlated with AHI,oxygen desaturation index (ODI) (r =0.285,P ＜ 0.05 and r =0.282,P ＜ 0.05),and negatively correlated with the lowest SaO2 and the average SaO2 (r =-0.257,P ＜ 0.05 and r =-0.259,P ＜ 0.05).The level of FIB was positively correlated with AHI,ODI (r =0.430,P ＜ 0.01 and r =0.311,P ＜0.01),and negatively correlated with the lowest SaO2 (r =-0.287,P ＜0.01).There were no correlations between ESR and sleep breathing parameters.Conclusion The levels of CRP,FIB and ESR are increased in OSAHS patients,and the levels of CRP and FIB are proportional to the severity of OSAHS,suggesting that OSAHS patients has systemic inflammatory response.
ABSTRACT
Objective@#To investigate the changes of C-reactive protein(CRP), fibrinogen(FIB) and erythrocyte sedimentation rate(ESR) in patients with obstructive sleep apnea-hypopnea syndrome(OSAHS), and their relationship with sleep respiratory parameters.@*Methods@#From June 2015 to August 2018, 70 OSAHS patients[observation group, apnea hypopnea index(AHI)≥5/h]and 20 persons in the snoring control group(control group, AHI<5/h) who received the monitor of polysomnography in the Pingxiang People′s Hospital were enrolled.The levels of CRP, FIB and ESR were measured and compared between the two groups.The correlations were analyzed between the levels of inflammatory markers and sleep breathing parameters.@*Results@#The two groups had no statistically significant differences in age, gender and BMI(all P>0.05). The CRP, FIB and ESR of the observation group were (4.5±3.0)mg/L, (3.3±0.9)g/L, (11.4±5.4)mm/h, respectively, which were significantly higher than those of the control group[(2.0±1.5)mg/L, (2.9±0.5)g/L, (7.6±2.6)mm/h](t=5.12, 2.68, 4.36, all P<0.05). The level of CRP was positively correlated with AHI, oxygen desaturation index(ODI)(r=0.285, P<0.05 and r=0.282, P<0.05), and negatively correlated with the lowest SaO2 and the average SaO2(r=-0.257, P<0.05 and r=-0.259, P<0.05). The level of FIB was positively correlated with AHI, ODI(r=0.430, P<0.01 and r=0.311, P<0.01), and negatively correlated with the lowest SaO2(r=-0.287, P<0.01). There were no correlations between ESR and sleep breathing parameters.@*Conclusion@#The levels of CRP, FIB and ESR are increased in OSAHS patients, and the levels of CRP and FIB are proportional to the severity of OSAHS, suggesting that OSAHS patients has systemic inflammatory response.
ABSTRACT
@#In order to improve the brain distribution of fibroblast growth factor 21(FGF21), TAT-FGF21 fusion protein was designed and its neuroprotective activity was investigated. The recombinant plasmid of pET28a-TAT-FGF21 was constructed and transformed into E. coli BL-21(DE3)sensitive bacteria. The TAT-FGF21 fusion protein was purified by Ni-NTA affinity chromatography column after IPTG induced expression. The SH-SY5Y cell damage model was induced by Aβ25-35, and the TAT-FGF21 fusion protein was used to intervene. The effects of Aβ25-35 and TAT-FGF21 induced on SH-SY5Y cell viability were determined using MTT method; DCFH-DA fluorescent probe was used to detect the intervention effect TAT-FGF21 on reactive oxygen species(ROS)generation induced by Aβ25-35 in SH-SY5Y cells; the effects of Aβ25-35 and TAT-FGF21 on mitochondrial membrane potential in SH-SY5Y cells were detected with JC-1 fluorescent probe. The results showed that TAT-FGF21 could improve the viability of SH-SY5Y cells, reduce the intracellular ROS production level of SH-SY5Y cells, and enhance the mitochondrial membrane potential of SH-SY5Y cells, which indicate that TAT-FGF21 could protect neurons on SH-SY5Y cell injury induced by Aβ25-35 through alleviating oxidative damage.