Histological and cytogebitical students on the role of alpha-lipoic acid against tetrachloroethane induced toxicity on testis and chromosomes of somatic and germ cells in mice

Tetrachloroethane [TTCE] a widely used industrial solvent causes damage to different organs via its free radicals production. The aim of this study was to detect the possible role of alpha-lipoic acid, being a natural antioxidant, against the TTCE-induced testicular and chromosomal toxicity in mice. Eighty male mice were classified into eight equal groups. Groups I, II and III were considered as control groups. Mice of Groups IV and V received alp ha-lipoic acid and TTCE respectively orally for 15 consecutive days. Mice of Groups VI, VII and VIII received three regimens of alph a-lipoic acid and tetrachloroethane [pre-, co-and post-tetrachloroethane,] at the same dose, route and duration for each. Three days following the last dose, half number of animals of all experimental groups was injected with cholchicine intraperitonealy to arrest the cell division. Two hours later, they were sacrificed for testicular histological examination and preparation of somatic and germ celI chromosomes. The other half number of the animals was sacrificed 35 days after the last dose of tested materials for sperm smear preparation. TTCE resulted in variable degrees of degenerative changes of time cells lining somniferous tubules, partial or complete absence of spermatids and mature sperms together with an increase in the mean value of sperm abnormalities. It also caused significant increase in the frequency of total chromosomal aberrations in both somatic and germ cells. Administration of alpha-lipoic acid in different manners resulted in a noticeable improvement of the degenerative testicular and chromosomal effects induced by TTCE. In conclusion, alpha-lipoic acid has an ameliorating role against TTCE-induced toxicity of testis and chromosomes

study of cardio protective effect of dipyridamole preconditioning on stressed isolated perfused rat hearts

Ischemic heart disease remains a leading cause of morbidity and mortality. The present study aimed at investigating the cardio protective effect of pharmacologic preconditioning with dipyridamole, an adenosine uptake inhibitor, on the variable facets produced by ischemia/reperfusion in isolated rat hearts previously stressed by isoproterenol infusion. Hearts were preconditioned by injecting rats i.p. with 4mgkg dipyridamole twice a day, 6 days / week, for 4 weeks. The control group received i.p injection of saline in a volume equivalent to that used to dissolve the drug. Isolated rat hearts were perfused for 3 min with isoproterenol, followed by 30 min of global ischemia and then 30 min of reperfusion. On exposing the heart to the stress of isoproterenol infusion followed by ischemia reperfusion insult, dipyridamole 1 was capable of improving recovery of cardiac muscle and decreasing the infarct size as compared with the control group. This could be explained by the present findings that dipyridamole preconditioning stimulated myocardial angiogenesis with marked increment in mitochondria1 number and size, and significantly de- creased MDA level to an extent that could be sufficient to exert significant cardio protection due to enhanced ventricular contractile functional reserve at pre-ischemic stag