ABSTRACT
Background: Recently, microplate almar blue assay has been used commonly in detecting gen related to tuberculosis drug resistance, which provides results after 5-7 days with lower cost compared to traditional methods. Objective: To evaluate the application of microplate almar blue assay for rapid detection of drug resistance of M. tuberculosis strains. Subject and Method: A microplate-based assay which uses Alamar blue reagent - an oxidation reduction dye (MABA), was used for the determination of the anti-tuberculosis drug (isoniazid-INH, rifampicine-RIF, streptomycine-STR and ethabuton=EMB) resistance of 96 M. tuberculosis strains isolated from Vietnamese patients in comparison to those obtained by conversional method. Result: MABA showed to have high sensitivity and specificity in testing the sensitivity to individual anti-tuberculosis drugs (from 82.4% for STR to 93.3% for - INH and from 82.5% for EMB to 98.4% for STR; respectively), as well as for the multi-drug resistant M.tuberculosis (86.4% of sensitivity), highly correlated with the result determined by proportion method. Conclusion: MABA reveals the advantage in shortening test time, in simple performance and lower cost compared with the conversional culture based methods.
Subject(s)
Drug ResistanceABSTRACT
The National development of global DOTS in Vietnam cured 90% of new patients with positive AFB from the tuberculosis and found 80% of estimated patients with tuberculosis the risk index of tuberculosis infection among Vietnamese was 1.7%. According to the estimation of world health organization, in 2001, the population in Vietnam was 79 million inhabitants. The newfound number of tuberculosis were 149,000 (189/100,000 inhabitants). The new estimated number of pulmonary tuberculosis with positive AFB were 67000 (85/100,000 inhabitants). The numbers of pulmonary tuberculosis with M. tuberculosis in the sputum were 53805 (80%). The number of pulmonary tuberculosis with positive AFB received DOTS were 53,561 (80.1%). The number of patients will be disease free were 48,892 (92.6%).
Subject(s)
TuberculosisABSTRACT
The objectives of national program of antituberculosis during 2001-2010 were to reduce to 50% of incidence and 50% of prevalence in order to reduced the morbidity rate, mortality rate of tuberculosis; early detect 75% of patients with the pulmonary tuberculosis with M. tuberculosis in the sputum; and reduce maximally the risks of drug resistance by successful treating 85% of patients under deployment of strategy of DOTS for 100% of tuberculosis patients