ABSTRACT
Noise, whether pleasant or not, is ever present in nature. Not much work can be done without noise, in the industries, fish market, social gatherings; it is constantly in the background. Thought it seems harmless loud and or prolonged exposure to noise can cause health problems to the populace. Noise, as a recognized stressogenic factor, has been postulated to stimulate the HPA axis leading to the release of stress hormones. The present study was designed to assess the changes occurring in the level of stress hormones [ACTH and Corticosterone] in albino rats when acutely [24 hrs] exposed to loud noise with subsequent ameliorating effects of benzodiazepine [Valium/ Diazepam] which will given to the rats to alleviate the symptoms of stress. Prospective Experimental Study. This study was conducted in the Department of Anatomy, JPMC, Karachi from Jan 2006 to June 2008. Three groups of 10 rats labeled A, B and C serving as control [no noise], noise stress [24hrs] and noise stress [24hrs] with diazepam [5mg/kg] respectively. The groups B and C were exposed to white noise at 100 dB. At the end of study the animals were sacrificed and their mean plasma concentration of ACTH and corticosterone were measured. A highly significant increase in mean plasma concentration of ACTH [150.90 +/- 14.71 pg/ml] and corticosterone [5.72 +/- 5.75 pg/ml] and corticosterone [1.75 +/- 0.25 micro/dl] concentration of control animals. This increase was significantly ameliorated by pre treatment of diazepam. Our study shows the protective role of diazepam in stress induced by noise
Subject(s)
Male , Animals, Laboratory , Noise , Corticosterone , Stress, Psychological , Adrenocorticotropic Hormone , Diazepam , Prospective Studies , RatsABSTRACT
Noise is an ever growing problem in modern society. Apart from its effect on hearing, behavior, and mood it also has a potent effect on immune system. Therefore the present study was designed to asses' effect of chronic noise stress on mantle zone [MnZ] of lymphoid follicle [LF] of spleen in albino rats. Two groups of twenty rats, each group labeled A and B were used; serving as control [no noise] and noise stress [30 days] respectively. Group B was exposed to noise stress of 100 dB for 30 day [3-4hrs/day]. Animals were sacrificed at the end of study, spleen was removed prepared, sectioned and stained with CD79a. A highly significant decrease in mean no of cell in MnZ of LF was observed [1 5747 +/- 87.82/mm[2]] in noise stressed group when compared with MnZ of control animals [16567 +/- 106/mm[2]]. There was also a highly significant increase in mean perimeter of nuclei observed in MnZ of stressed group [2.74 +/- 0.009pm] when compared with rats of control group [2.68 +/- 0.01microm]. Our study concludes that noise stress induced release of glucocorticoid can significantly decrease number of B lymphocytes in MnZ of LF of spleen in albino rats
ABSTRACT
Noise is an ever growing problem in modern society. Apart from its effect on hearing, behavior, and mood it also has a potent effect on immune system. Therefore the present study was designed to asses effect of chronic noise stress on mantle zone [MnZ] of lymphoid follicle [LF] of spleen in albino rats. Two groups of twenty rats, each group labeled A and B were used; serving as control [no noise] and noise stress [30 days] respectively. Group B was exposed to noise stress of 100 dB for 30 day [3-4hrs/day]. Animals were sacrificed at the end of study, spleen was removed prepared, sectioned and stained with CD79a. A highly significant decrease in mean no of cell in MnZ of LF was observed [15747 +/- 87.82/mm[2]] in noise stressed group when compared with MnZ of control animals [16567 +/- 106/mm[2]]. There was also a highly significant increase in mean perimeter of nuclei observed in MnZ of stressed group [2.74 +/- 0.009microm] when compared with rats of control group [2.68 +/- 0.01microm]. Our study concludes that noise stress induced release of glucocorticoid can significantly decrease number of B lymphocytes in MnZ of LF of spleen in albino rats