ABSTRACT
This research was aimed to study the effects of oral administration of Camellia sinensis L. on the testicular and body weights of adult Wistar rats for short and long time periods. The adult Wistar rats were divided into 3 groups [A, B and C]. Every group had ten rats. Green tea extract 0.692% [w/v] was given to groups A and B on daily basis. The extracts were prepared fresh and given for a period of ten and thirty days, respectively, while distilled water was given to the group C rats only. The adult Wistar rats were sacrificed on eleventh and thirty-first day of experiment for the particular groups. The testes were dissected out cautiously, free from the supporter tissues and weighed to the adjacent 1 mg. There is no significant difference in the body weight in all 3 groups. Moreover, it was observed that Wistar rat's testicular weight was considerably increased in group B but no major changes were seen in group A. Our results indicated that green tea when given for short period of time may be effective to the testes but has no consequence on Wistar rat's body weight. However, it is indistinct if these alterations are reversible
Subject(s)
Animals, Laboratory , Herbal Medicine , Testis , Rats, Wistar , Body Weight , Plant ExtractsABSTRACT
Compare the anti ulcer effect of Methanolic Neem [Azadirachta indica, A Juss, Meliaceae] Leaf Extract [NLE] and Neem compound Nimolicin on gastric mucosa of albino rats. Azadiradione also called Nimolicine coded as NC has been studied for its anti insect effect but anti ulcer effect has never been studied. Experimental study. This study was carried out at the Pharmacy and Physiology Department of Baqai Medical College for duration of two years. Gastric ulcers in albino rats were induced in group-1 [check group] by a single oral dose of 1 ml 100% ethanol. After 24 hours the treatment was started. Group-2 was treated with oral administration of pea nut oil 1 ml/day for 5 days [control of the treated group]. Group-3 was treated with NLE [1 ml/day for 5 days] and Group-4 with NC 1% [1 ml/day for 5 days]. The healing effects of neem were compared to oral administration of anti ulcer drugs ranitidine [50mg/kg daily for 5 days] and omeperazole [2.5 mg/kg daily for 10 days]. Histopathology of the stomach was performed to confirm the presence or healing of ulcers. Ulcers were scored and indexed on the basis of histopathology. Ranitidine had the highest ulcer inhibition of 84%. NLE proved to be better than omeperazole by showing an ulcer inhibition of 82 % compared to 73% ulcer inhibition of omeperazole. NC showed least anti ulcer activity with an ulcer inhibition of only 69%. HPLC was performed to show the tissue concentration of NC, omeparazole and ranitidine showing their retention time, area and concentration compared to their controls. It is concluded that NLE proved to be better anti ulcer agent as compared to NC and can be used as an anti ulcer drug after clinical trials