ABSTRACT
Enzyme inhibition is a significant part of research in pharmaceutical field in view of the fact that these studies have directed to the innovations of drugs having remarkable performance in diverse physiological conditions. The present study was aimed to assess urease and lipoxygenase inhibitory activity of weight reducing tablets. For evaluating the urease activity indophenol method was employed using Thiourea as the model urease inhibitor. The lipoxygenase inhibition was evaluated by measuring the hydroperoxides produced in lipoxygenation reaction using a purified lipoxygenase with lionoleic acid as substrate. When formulation of the weight reducing tablets was compared at various concentrations [50, 100 and 500microg/ml]. The antiurease activity and lipoxygenase inhibition activity increased in a dose dependent manner. The formulations under test have an excellent antiurease and lipoxygenase inhibition potential and prospective to be used in the cure of a variety of complications associated with the production of urease and lipoxygenase enzymes
ABSTRACT
Antioxidants are used as an influential counteractive measure in opposing the generation of reactive oxygen species. The current study was carried out to investigate antioxidant potential and reducing capability of developed weight reducing tablet formulation. When tablets were evaluated at concentrations of 50, 100 and 500 micro g/ml, antioxidant activity improved in a dose depending way just similar to standard Butylated hydroxyl anisol [BHA]. For evaluation of reducing ability the formulation under test evaluated at concentrations of 50, 100 and 500micro g/ml and it was observed that formulation contain good reducing capability and possess considerable activity to scavenge super oxide radicals. In-vitro analysis of weight reducing tablets formulation showed considerable antioxidant and reducing capacity that will be supportive in averting the development of a variety of oxidative stress-related diseases