ABSTRACT
Background: The presence of large number of tonsillectomy surgeries and also the possibility of tonsillar colonization by Helicobacter pylori as the causative agent of tonsillar hypertrophy make it necessary to investigate any possible correlation between the presence of this bacterium and the occurrence of tonsillar hypertrophy
Objective: To investigate the frequency of Helicobacter pylori in chronic tonsillitis patients
Methods: Scorpion real-time PCR was performed on 103 archived paraffin-embedded tonsillar samples collected from patients with tonsillar hypertrophy following tonsillectomy operation at ENT ward of Qods Hospital [Qazvin University of Medical Sciences], Qazvin [Iran] during 2008-2009
Findings: H. pylori DNA was present in 21.35% of total specimens
Conclusion: Although the existence of H. pylori in tonsillar tissue samples of patients with tonsillar hypertrophy is controversial however, it seems that the method by which the laboratory investigation is made may influence the results as the more sensitive and specific scorpion real-time PCR assay showed the tonsils could be considered as important reservoir of H. pylori
ABSTRACT
Introduction: Tuberculosis is one of the oldest diseases of human. Before application of the molecular methods, because of the long duration of diagnostic- microbiologic procedures [culture and biochemical tests], the diagnosis and beginning of the therapy required experience and clinical intelligence. The diagnostic microscopic assessment methods of the smear and culture of the specimens have their own limitations, one of which is the slow growth of mycobacteria [4-8 weeks] in the culture media.Nevertheless, achievement to the more rapid diagnostic procedures is the main aim in this regard. From the beginning of the last decade, PCR and other more rapid diagnostic assays for the diagnosis of the infections due to Mycobacterium tuberculosis have been used
Objective: Since no laboratory diagnostic procedure has been known as the alternative to the culture, this study has been done for determination of sensitivity, specificity, positive and negative predictive values of the PCR test in comparison to the culture in this center
Materials and Methods: In this study, 169 specimens from the patients with positive smears were cultured on the Lowenstein- Jensen media and after subculturing and related biochemical tests, the results obtained were compared with PCR test results [with IS 6110 target and Dra, Drb primers]
Results: Obtained data showed that from 169 smear positive cases, there were 150 positive cultures and after doing biochemical tests, 86 cases were detected as Mycobacterium tuberculosis. According to these results, sensitivity, specificity, positive and negative predictive values of the PCR test in this center were 91.86%, 96.87%, 97.53% and 89.85% respectively
Conclusion: Concomitant use of PCR with culture and biochemical tests is recommended
ABSTRACT
Background and Objective: urinary tract infections [UTI] are regarded as one of the most common infectious diseases. A remarkable percent of urinary tract infections are asymptomatic. In some cases of urinary tract infections, significant bacteriuria is not present. One the etiologic agents of culture negative genitourinary tract infections, which can be transmitted through intercourse, is Chlamydia Trachomatis. On the basis of high incidence of negative results of urine culture [Up to 60%] in patients suffering from UTI referring to Bou-Ali infectious hospital and taking into consideration the importance of genitourinary Chlamydial infections, we decided to study prevalence of Chlamydia Trachomatis in urine sample of patients with UTI referring to this hospital
Materials and Methods: this research was a descriptive study on the 320 patients referring to Bou-Ali infectious hospital in Zahedan, which were chosen by non-randomized sampling. One early morning urine sample was taken from these patients. After centrifugation, sediment of samples was used as antigen for Chlamy-check-I ELISA kits
Results: out of 320 urinary specimens, 95 specimens [29.69%] were positive and 225 specimen [70.31%] were negative for Chlamydia Trachomatis. From 95 positive samples, 43 person [45.2%] were male and 52 person [54.73] were female. In both sexes the highest percent was related to 20-29 years old group
Conclusion: in accordance with above reported prevalence rate and bearing in mind the consequences of inappropriate treatment of Chlamydial infections [PID, infertility, extra uterus pregnancy, epididiomitis] the importance of precise treatment of Chlamydial infection and necessity of providing laboratory facilities for accurate and rapid diagnosis of Chlamydial infections in the area with high incidence of culture negative urine specimens is recommended