ABSTRACT
Background and Purpose: Leptin is one of the most important proteins involved in lipid metabolism and energy homeostasis but the effect of acute exercise on leptin has been less investigated. The purpose of the present study was to investigate the effect of a one-session exercise on tissue concentration and gene expression of leptin in rats
Methods and Materials: In this experimental study, 24 male Wistar rats were housed in a standard environment, and were randomly divided into two groups [Control and Experimental]. The exercise was running on a treadmill for 120 min [18 m/min]. Immediately, 2 and 24 hours after exercise the rats anesthetized, blood, fat tissue and soleus were taken. Leptin concentration in the muscular, fat and blood were measured. Also, leptin gene expression was investigated with RT-PCR in tissues. Data were analyzed in SPSS 16 using repeated measures ANOVA [P<0.05]
Results: The results showed that leptin gene expression significantly increased in muscles 2 hours [[51%] from 169/15 +/- 10/36 to 349/13 +/- 112/71] and 24 hours [[48%] from 185/14 +/- 8/21 to 359/01 +/- 14/77], and in adipose tissue 24 hours [[49%] from 181/69 +/- 21/42 to 361/66] [P<0.05]. However, leptin concentration did not change neither in adipose tissue nor in muscle nor plasma [P>0.05]. Also, there was no significant differences in plasma leptin, glucose and insulin between the two groups across the three times of mesurement [P>0.05]
Conclusion: Acute exercise can increase leptin gene expression in muscle and fat tissues. However, exercise with longer duration and higher volume may appear to be more effective
ABSTRACT
Background and Purpose: Obesity is associated with various metabolic and inflammatory impairments, the effects of which have not been sufficiently studied. Therefore, the purpose of this study was to examine the influence of an 8-week strength training and aerobic training program and a 10-day detraining on lipid profile and CRP in obese girls
Methods and Materials: In this experimental study, thirty-six obese female university students were voluntarily recruited and randomly assigned into three groups: control [n=11], aerobic training or AT [n=12] and strength training or ST [n=13]. ST and AT groups trained for 8 weeks, 4 times/week, 60 min/session, with 60 to 70% of 1 repetition maximum for ST, and 65 to 75% of HRmax for AT. Blood samples were taken at baseline, 24 hours after the last exercise session and after 10 days of detraining on fasting state. Data were analyzed in SPSS 16 using repeated measure analysis of variance [RM-ANOVA]. The alpha level was established at P<0.05
Results: After an 8-week training program, TG decreased in AT and ST groups [4.2 % and 6.6 %, respectively] but the change was not significant [P>0.05] Also, no significant differences were found between the three groups [P>0.05]. TC also decreased in AT and ST groups [9% and 19%, respectively]; however, the change was not significant [P>0.05]. After training no significant changes in HDL, HDL2, HDL3, LDL, and CRP were observed [P>0.05]. Ten days of detraining also did not result in any significant alterations in the lipid profile or CRP [P>0.05]
Conclusion: Eight weeks of aerobic and strength training have no significant effect on the lipid profile and CRP in obese girls