A Case of Solitary Morphea Profunda / 대한피부과학회지

Morphea is a benign, cutaneous form of scleroderma and morphea profunda is one of its six clinical variants. The solitary morphea profunda is characterized by thickening and hyalinization of subcutaneous tissue and fascia with more dense inflammatory cell infiltrate and paravertebral predominant site. We present a case which showed characteristic histologic findings such as hyalinization of collagen fiber, lymphocytic and plasma cell aggregates and infiltrations.

Scanning Electron Microscopic Examination of Dermatophytes / 대한의진균학회지

BACKGROUND: It is important to understand the morphologic characteristics for the identification of a microorganism. Electron microscopy has contributed a great deal to the field of mycology. Scanning electron microscope (SEM) has become a useful tool to study three-dimensional images of fungi. Vacuum dessicator aqueous fixation is a useful sample preparation technique for SEM examination of fungal specimens without surFace distortions. OBJECTIVE: This study was done to observe the three-dimensional structures of dermatophytes preserving their natural growth characteristics. METHODS: Trichophyton rubrum, T. mentagrophytes, Microsporum canis, M. gypseum, and Epidermophyton floccosum were isolated and cultured on Sabourd agar, corn meal agar, or rice agar plate. Vacuum dessicator aqueous fixation was utilized as p preparation technique for SEM examinations of fungi. RESULTS: Vacuum dessicator aqueous fixation led us to examine the aerial mycelia including conidia of the dermatophytes under SEM. The five species of dermatophytes showed their own characteristic structures especially on microconidia and macroconidia. CONCLUSION: The use of ultrastructural research has made it possible to identify the species of fungi and can be expanded and improved along with the development of SEM and specimen preparation techniques.