ABSTRACT
The study was performed to investigate the effect of marbofloxacin [2mg/kg body weight /day] on some immunological parameters in 9 sheep [35-40 kg. body weight] immunized with live attenuated sheep pox vaccine. Sheep were allocated into 3 equal groups. The first group was left as control; the second group was injected intramuscular [I/M] by marbofloxacin [2mg/kg body weight/day for 5 successive days] then vaccinated by intradermal [I/D] injection of 0.5 ml sheep pox vaccine/sheep. The third group was vaccinated with sheep pox vaccine only. The data revealed stimulation in the level of superoxide anion produced by neutrophil represented by detection of cytochrom C reduction level in the 2[nd] group [p<0.05] on the 1[st] day post vaccination, while there was significant depression in the level of lysozyme in the 1[st] two days post vaccination. The level of serum nitric oxide was not affected in the three groups. The lymphocytes transformation depressed significantly [p<0.05] in the 1[st] week post vaccination in the 2[nd] group as compared with the other two groups. It was concluded that marbofloxacin in its therapeutic level induced initial immunostimulant effect on the neutrophil followed by a transient immunosuppressive effect on other cells as macrophage when used before vaccination with live vaccine