ABSTRACT
Background: Radiotherapy has many side effects on fertilization in young women. Radiation can lead to ovarian failure in women who underwent abdomen or pelvic radiotherapy
Objective: This study helps us to investigate ovarian response of NMRI female mice to ovarian stimulating hormones [PMSG, HCG] after whole-body gamma irradiation
Materials and Methods: 45 pregnant mice were divided into two groups of control and experimental. The experimental group was classified into three sub-groups: Irradiation group [2 or 4Gy],Superovulation group [10 or 15IU],and superovulation and gamma-radiation group [2Gy and 10IU, 2Gy and 15IU, 4Gy and 10IU,4Gy and 15IU]. Female mice were killed and embryos were removed from oviduct .The number of embryos cells counted and the quality of them was evaluated in each group. Kruskal-Wallis test and Mann-Whitney test were used to analyze the data
Results: There was a significant difference in the number of 2-4 cells grade D embryos in 2Gy and 15IU group compared with control and 2Gy groups [p=0.01], and the number of embryos in 4Gy group was more than in 10IU and 15IU [p=0.03] and 2Gy and 15IU groups [p=0.01]. It was more significantly embryos in 4Gy and 15IU group compared to 2Gy and 15IU group [p=0.01].In addition There were no significant differences in the number of 2-4 cells grades A, B and C embryos and also number of 4-8 cells grades A, B and C, D embryos in groups
Conclusion: The concurrent use of ovulation stimulating hormones and gamma rays ameliorates this problem of drastic decrease in number of living embryos due to whole-body irradiation