ABSTRACT
Using aspirin, heparin, or both in women with unexplained recurrent miscarriage could be useful, because this problem might be initiated by thrombosis in decidual vessels. To investigate the association between thrombophilia and unexplained recurrent miscarriage and to evaluate the efficacy of anticoagulant treatment. In this quasi experimental, we enrolled 520 women, who had a history of recurrent miscarriage. Two hundred fifty two women with unexplained recurrent miscarriage were assigned to receive aspirin [80 mg daily] for two month before pregnancy and after confirmation of a viable pregnancy until 36 weeks of gestation or receive aspirin, as the same, plus heparin [5000 unit twice a day] subcutaneously after confirmation of viable pregnancy until 4 weeks after delivery. Type of medication was chosen for each woman according to number of abortion and age. Live-birth rates did not different significantly among the two study groups. The proportions of women who gave birth to a live normal infant were 74.5% in the group receiving aspirin plus heparin [combination-therapy group] and 79.8% in the aspirin group. Live-birth rates did not different significantly among the two study groups. So, using aspirin or aspirin plus heparin did not change pregnancy rate in these patients. Using aspirin is easier than injecting heparin which should be chosen case by case
ABSTRACT
Background: meiotic genes are very important candidates for genes contributing to female and male infertility. Mammalian MutL homologues have dual roles in DNA mismatch repair [MMR] after replication errors and meiotic reciprocal recombination. The MutL homologs, MLH1 and MLH3, are crucial for meiotic reciprocal recombination and human fertility. In this study the functional polymorphisms of MLH3C2531T was investigated in Iranian women with unexplained infertility
Objective: investigating the association between a common SNP [single nucleotide polymorphism] C2531T in the MLH3 gene and female infertility
Materials and Methods: in total, 105 women with unexplained infertility as case group and 100 women with at least one child and no history of infertility or abortion as controls were recruited for this association study. The MLH3 C2531T polymorphism was tested by tetra-amplification refractory mutation system-PCR [4P-ARMS-PCR] method
Results: the MLH3 2531C and T alleles frequencies were 43.33% and 56.67% among infertile patients, and 61.5% and 38.5% among normal controls, respectively. In the patient and control subjects the CC [Pro 844 Pro] genotype frequency ofMLH3 C2531T was 4.76% and 25%, the CT [Pro 844 Leu] genotype was 77.15% and 73%, and the TT [Leu 844 Leu] genotype was 19% and 2%, respectively [p=0.0001]
Conclusion: the presence of the polymorphic allele T leads to an increased risk of 2.09 times [OR=2.09, 95% CI=1.38-3.16; p=0.0001] for developing infertility in relation to the control group. Therefore, our data suggest that the MLH3 C2531T polymorphism can be associated with the risk of unexplained infertility in Iranian women