ABSTRACT
The present article reviews the challenges and hurdles in the development of an analytical method for aminoglycosides(AG). The article emphasizes on the attempts made to develop analytical methods based on HPLC and othersophisticated techniques, such as LC-MS, radioimmunoassay, microbial assay, enzyme linked immunosorbentassay (ELISA), extractive colorimetry, anion-exchange chromatography with pulsed amperometric detection, highperformance thin layer chromatography, densitometry, and microbial agar diffusion assay. The various media mostlyused for the in vitro as well as in vivo estimation of AG by HPLC and LC-MS are heptafluorobutyric acid, ammoniumacetate, ammonium formate and formic acid. Estimation of AG by radioimmunoassay and ELISA can be suitably doneby using TRIS-HCl and saline phosphate buffer. The buffer media used for ex vivo analysis mostly include MEM,TRIS and saline phosphate. The presence of AG in food from the animal sources, water bodies, and its prolongedexposure may result in serious health issues. The present article outlined the various sensitive, robust and preciseanalytical techniques for the estimation of the various aminoglycosides in many sources, and discussed the hurdlesfaced during the development of the analytical techniques.