ABSTRACT
Objective: To determine the role of glutathione S-transferase [GST] isoenzyme polymorphisms as susceptibility factors in patients with psoriasis in a Turkish cohort
Subjects and Methods:In this case-control study, 105 patients with plaque-type psoriasis and 102 healthy controls were recruited from the dermatology outpatient clinics of two university hospitals. Genomic DNA was extracted from whole blood using a DZ DNA isolation kit. Multiplex PCR was used to determine GSTM1 and GSTT1 polymorphisms in the isolated DNAs
Results:Of the 150 patients with psoriasis, 83 [79%] were identified with the GSTT1 genotype and 22 [21%] with the null genotype. Of the 102 patients in the control group, 69 [67.6%] subjects were identified with the GSTT1 genotype and 33 [32.4%] with the null genotype. There was no significant difference between the patient and control groups [p = 0.063]. Regarding the GSTM1 polymorphism, 54 [51.4%] patients were identified with this genotype and 51 [48.6%] with the null genotype; in the control group, 50 [49%] were identified with this genotype and 52 [51%] with the null geno- type. Again there was no statistically significant difference between the groups [p = 0.957]
Conclusion:In this Turkish cohort of patients with psoriasis, neither GSTT1 nor GSTM1 polymorphisms were associated with disease susceptibility. Larger studies with a wider range of GST isoenzyme are needed
ABSTRACT
OBJECTIVE: This work investigates the role of glutathione S-transferase M1 (GSTM1), glutathione S-transferase T1 (GSTT1), and glutathione S-transferase P1 (GSTP1) enzymes and polymorphisms, which are found in phase II detoxification reactions in the development of cervical cancer. METHODS: This study was conducted with 46 patients diagnosed with cervical cancer and 52 people with no cancer history. Multiplex PCR methods were used to evaluate the GSTM1 and GSTT1 gene polymorphism. However, the GSTP1 (Ile105Val) gene polymorphism was studied using a PCR-RFLP method. The patient and control groups were compared using a chi-square test with p0.05). CONCLUSION: Our results demonstrate that GSTT1, GSTM1, and GSTP1 polymorphisms are not associated with cervical cancer in Turkish patients.