Multidrug resistance reversal activity of extract and a rare dimeric naphthoquinone from Diospyros lotus

A dimeric naphthoquinone namely dihydrodyspyrole R [1] was purified once more from Diospyros lotus. Dihydrodyspyrole R and chloroform fractions were evaluated for their effects on the reversion of multidrug resistance [MDR]. The compounds [1] and extract exhibited promising MDR reversing effect in a dose-dependent manner against mouse T-lymphoma cell line. Molecular docking of compound 1 revealed the correlation between in-silico with in-vitro results. The molecular docking results showed that compound 1 is bind closely where co-crystal ligand of P-gp is present. But usually, computational investigation predicts that, if a compound gives lesser score then compound will exhibit good activity. Hence, the docking scores of compound 1 are the near to the Rhodamine. It is conclude that there are certain important structural features of compound 1which are responsible for the inhibiting potency of P-gp from mice. The computational Petra/Osiris/Molinspiration [POM] analysis confirms the possibility of use of compound 1 without side effect or less toxicity risks

In vivo antinociceptive and muscle relaxant activity of leaf and bark of Buddleja asiatica L.

The current study was designed to assess the antinociceptive and skeleton muscle relaxant effect of leaves and barks of Buddleja asiatica in animal models. In acetic acid induced writhing test, pretreatment of ethanolic extract of leaves and barks evoked marked dose dependent antinociceptive effect with maximum of 70% and 67% pain relief at 300mg/kg i.p. respectively. In chimney test, the ethanolic extract of leaves and barks evoked maximum of 66.66% and 53.33% muscle relaxant effect after 90min of treatment at 300mg/kg i.p respectively. In traction test, the ethanolic extract of leaves and barks caused maximum of 60% and 73.33% muscle relaxant effect after 90min of treatment at 300mg/kg i.p respectively. In short, both leaves and barks demonstrated profound antinociceptive and skeleton muscle relaxant effects and thus the study provided natural healing agents for the treatment of said disorders

Antioxidant and nutraceutical value of wild medicinal Rubus berries

Nutritional quality and antioxidant capacity of three edible wild berries [Rubus ellipticus Smith, Rubus niveus Thunb, Rubus ulmifolius L.] from Lesser Himalayan Range [LHR] were evaluated. Their edible portion was assayed for moisture, fats, ash, carbohydrates, proteins, fibers, essential minerals [Ca, P, Mg, K, Na, Cl, S, Mn, Zn, Fe, Cu, Se, Co, Ni] and DPPH free radical scavenging activity was applied to determine the antioxidant potential. The fruit of Rubus ulmifolius L. [blackberry] possessed the highest values of energy [403.29 Kcal], total protein [6.56g/100 g], Nitrogen [N] content [1500mg/100g], K [860.17mg/100g], Ca [620.56mg/100g], Zn [17.509mg/100g] and the strongest antioxidant activity [98.89% inhibition]. While the raspberries [Rubus ellipticus Smith, Rubus niveus Thunb.] exhibited more significant contents of dietary fiber [5.90g/100g], carbohydrates [86.4 g/100 g] and Fe [4.249mg/100g]. Significant variation was observed among the tested samples in all the investigated features. The combination of bio elements and active antioxidants clearly showed the applicability of these berries as a nutraceutical supplement