ABSTRACT
OBJECTIVE:To compare the contents of osthole and columbianadin in Argelica. pubescens in Wushan county, Wuxi county in Chongqing and other domestic areas in china. METHODS:HPLC was performed on the column of Shiseido Cap-cell Pak C18 with mobile phase of acetonitrile-water(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 330 nm,column temperature was 30 ℃ and volume injection was 10 μl. RESULTS:The linear range was 0.015 1-0.301 6 mg/ml(r=0.999 9) for osthole and 0.016 7-0.334 4 mg/ml(r=0.999 9) for columbianadin;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 98.46%-106.11%(RSD=2.34%,n=9)and 98.24%-102.69%(RSD=1.29%,n=9), respectively. CONCLUSIONS:The method is simple and good reproducibility,and and can be used for the contents determination of osthole and columbianadin in A. pubescens. The contents of osthole and columbianadin in A. pubescens of Wushan county in Chongqing are obviously higher than Wuxi county and other domestic areas in China.
ABSTRACT
AIM:To establish the quality standard for Tongbianling Capsule(Folium Sennae,Radix Angelicae sinensis,Herba Cistanches). METHODS: Folium Sennae,Radix Angelicae sinensis of Tongbianling Capsuls were identified by TLC.Cisttanoside and echinacoside were determined by HPLC together.The HPLC system consisted of shim-pack c_(18) column(4.6 mm?150 mm,5 ?m),acotonitrile-methanol-0.2% pure phosphoric water solution(7∶10∶83) mixture as mobile phase,flow rate of 1.0 mL/min,detection wavelength at 334 nm. RESULTS: The TLC method was suitable for the identification of salidroside and ptyrosol with good reproducibility.The quantitative evaluation of cistanoside had the linear range of 0.114 9-2.298 ?g,the average recovery was 98.2%(RSD=1.70%,n=6).The quantitative evaluation of echinacoside had the linear range of 0.147 9-2.958 ?g,the average recovery was 96.8%(RSD=1.53%,n=6). CONCLUSION: The TLC and HPLC method is found to have good stability and reproducibility for Tongbianling Capsule.