ABSTRACT
<p><b>OBJECTIVES</b>In addition to having health-promoting effects, exercise is considered to induce oxidative stress. To clarify whether increased oxygen consumption during exercise induces oxidative stress, we investigated the effects of aerobic exercise and anaerobic exercise on a series of oxidative damage markers.</p><p><b>METHODS</b>One group of subjects performed aerobic exercise and another group performed anaerobic exercise with similar workloads, but with different levels of oxygen consumption. Blood and urine samples were collected before, immediately after, and 3, 9, and 24 h after exercise. Serum uric acid (UA) and creatine phosphokinase were evaluated. As markers of oxidative damage to lipids, proteins and DNA, we evaluated serum 4-hydroxy-2-nonenal, urinary F(2)-isoprostanes, serum protein carbonyls, and leukocyte 8-hydroxydeoxyguanosine.</p><p><b>RESULTS</b>Oxygen consumption was significantly greater during aerobic exercise. Although UA level increased immediately after aerobic exercise and decreased thereafter, UA level did not change after anaerobic exercise. The two types of exercise had significantly different effects on the change in UA level. After anaerobic exercise, the levels of 8-hydroxydeoxyguanosine and 4-hydroxy-2-nonenal significantly increased at 24 h and 3 h, respectively. The levels of creatine phosphokinase and F(2)-isoprostanes decreased after exercise. The two types of exercise caused no apparent significant differences in the levels of these biomarkers.</p><p><b>CONCLUSION</b>The findings suggest that similar workloads of anaerobic exercise and aerobic exercise induce reactive oxygen species (ROS) differently: aerobic exercise seems to initially generate more ROS, whereas anaerobic exercise may induce prolonged ROS generation. Although more oxygen was consumed during aerobic exercise, the generated ROS did not induce significant oxidative damage. Oxygen consumption per se may not be the major cause of exercise-induced oxidative damage.</p>
ABSTRACT
Objectives: In addition to having health-promoting effects, exercise is considered to induce oxidative stress. To clarify whether increased oxygen consumption during exercise induces oxidative stress, we investigated the effects of aerobic exercise and anaerobic exercise on a series of oxidative damage markers. Methods: One group of subjects performed aerobic exercise and another group performed anaerobic exercise with similar workloads, but with different levels of oxygen consumption. Blood and urine samples were collected before, immediately after, and 3, 9, and 24 h after exercise. Serum uric acid (UA) and creatine phosphokinase were evaluated. As markers of oxidative damage to lipids, proteins and DNA, we evaluated serum 4-hydroxy-2-nonenal, urinary F2-isoprostanes, serum protein carbonyls, and leukocyte 8-hydroxydeoxyguanosine. Results: Oxygen consumption was significantly greater during aerobic exercise. Although UA level increased immediately after aerobic exercise and decreased thereafter, UA level did not change after anaerobic exercise. The two types of exercise had significantly different effects on the change in UA level. After anaerobic exercise, the levels of 8-hydroxydeoxyguanosine and 4-hydroxy-2-nonenal significantly increased at 24 h and 3 h, respectively. The levels of creatine phosphokinase and F2-isoprostanes decreased after exercise. The two types of exercise caused no apparent significant differences in the levels of these biomarkers. Conclusion: The findings suggest that similar workloads of anaerobic exercise and aerobic exercise induce reactive oxygen species (ROS) differently: aerobic exercise seems to initially generate more ROS, whereas anaerobic exercise may induce prolonged ROS generation. Although more oxygen was consumed during aerobic exercise, the generated ROS did not induce significant oxidative damage. Oxygen consumption per se may not be the major cause of exercise-induced oxidative damage.
Subject(s)
Exercise , Exercise , Oxidative Stress , Oxygen ConsumptionABSTRACT
We investigated the effects of underwater exercise plus hot-spring bathing on middle aged people.<br>Twenty-five subjects (mean age: 59.6±8.9 years) were divided into three groups (A, B, and C) and examined. Group A (three males, seven females) was subjected to underwater exercise and hot-spring bathing. Group B (eight females) was subjected to underwater exercise and fresh water bathing. Group C (seven females) was a control group (no exercise and no bathing).<br>Group A and B performed underwater exercise consisting of underwater walking, basic exercise, and swimming for 70 minutes. Group A was then subjected to hot-spring bathing (sodium bicarbonate spring, PH 8.0, 39°C) for 20min, and while Group B was subjected to fresh-water bathing (30°C).<br>Blood pressure was measured and blood tests were conducted early in the morning on the day of exercise plus bathing and on the following day. Further, Profile of Mood Status (POMS) tests were also conducted on the day following the exercise with bathing and data were compared.<br>The findings were as follows.<br>1) On the day following the exercise plus bathing, group A (underwater exercise plus hot-spring bathing) had significantly lower diastolic blood pressure, total cholesterol, CD4, red blood cells, hematocrit and total protein when compared with values before treatment (p<0.05).<br>2) After the exercise plus bathing, group A (underwater exercise plus hot-spring bathing) had significantly lower levels of depression-dejection, anger-hostility and confusion in POMS tests when compared with those of Group C (control group, no exercise and no bathing) (p<0.05).<br>We concluded that underwater exercise plus hot-spring bathing has good effects on the health of middle aged people.