ABSTRACT
OBJECTIVE: To determine the PON1 activity and phenotype distribution in a Thai population. STUDY DESIGN: Prospective Descriptive study. MATERIAL AND METHOD: Between October 2001 and April 2002, 160 healthy Thai individuals aged 20-74 years were assessed for PON1 activity and the phenotype distribution by using dual substrate method. RESULTS: The means +/- SD of basal, salt-stimulated paraoxonase and arylesterase activities were 239.7 +/- 83.9 nmol/min/mL 555.2 +/- 222.2 nmol/min/mL and 147.6 +/- 33.8 micromol/min/mL respectively. The authors observed a wide interindividual variability up to 6.9-fold for paraoxonase activity and 4.6-fold for arylesterase activity. The authors found a range of ssPON/ARE ratio from 1.04 to 7.05 and three distinctive phenotype modals of AA (1.04-2.25), AB (2.44-4.29), and BB (4.53-7.05) with frequencies of 14.4% (AA), 51.9% (AB), and 33.7% (BB). The authors also observed the association of sex with lipid parameters and PON1 activity. CONCLUSION: The distribution of PON1 phenotype in Thais is clearly trimodal with high frequency in BB phenotype.
ABSTRACT
The level of fructosamine was determined in the serum of 278 individuals ranging in age from 20 to 80 years who passed a physical check-up. The 130 males and 148 females had mean ages + standard deviation of 44.0+15.0 and 40.3+12.0 years, respectively. Serum fructosamine concentration was assayed using nitroblue tetrazoleum (NBT) reduction in alkaline medium. The reference values of serum fructosamine were 226 µmol/L to 296 µmol/L (Mean = 260.7, SD = 17.6 µmol/L). The serum fructosamine is unaffected by sex. Effect of food intake on fruc tosamine level has been carried out on a group of healthy subjects (n = 36). The means and SDs of serum fructosamine at eight-hour fasting, two-hour post-prandial and non-fasting specimens were studied and values of 269.4+17.9, 269.3+14.8 and 268.6+15.3 µmol/L respectively, were obtained. Results reveal that food intake has virtually no effect on fructosamine values (Analysis of variance p>0.05). Parallel assays of 46-paired sera was Y = 17.5 + 0.95 X, whereas X and Y were serum and plasma fructosamine levels, respectively. Values of plasma fructosamine were statistical significantly difference from those of serum (p<0.001). A small discrepancy of 1.9 percent was observed. Thus, whenever plasma is used as sample for fructosamine test, a slightly higher value of fructosamine would be obtained.