Immunohistochemical study of the prevalence of EBV- antigen in nasopharyngeal carcinoma

A broad range of neoplasms can arise in the nasopharynx derived from epithelial, lymphoid, mesechymal, neurogenic, and rarely from embryonic remnant. These tumours have a long latent period with few primary symptoms which often lead to delay in there diagnosis. This retrospective study was designed for highlighting the challenges involved with the recognition of the nature of nasopharyngeal carcinoma, their classification, and correlation of the available clinical data with histopathological and immunohistochemical diagnosis and the prevalence of EBV infection among the studied cases of NPC. The present work was carried out on 23 cases referred as biopsy from nasopharyngeal tumour to Pathology Department, Faculty of Medicine Tanta university, Tanta Cancer center, the private laboratory during the last five years started from January 2002.and ended on December 2006 .Tissue specimens were all obtained by bunch and excision biopsy. Available clinical data were tabulated and analysed. All cases were subjected to routine histopathological examination and immunohistochemical study for EBV-LMP1. In the present study UNPC was more common [69.6%] than keratinizing NPC [30,4%]. The former occurred in younger patient [9/16], meanwhile the latter occurred in older individuals [5/7], NPC was common in male [60.9%] and bimodal distributions of cases were detected. The diagnosis of UNPC in most cases could be established through the presence of atypical epithelial cells and the lack of cytological atypia among reactive lymphocytes, as well as by immunohistochemical examination for EBV-LMP1. In the present study IHC was employed on paraffin embedded specimens of all the studied cases of NPC using of EBV-LMP1 reactivity. Accordingly, it was found that positive strong cytoplasmic and /or membranous immunorectivity for EBV-LMP1 was evident in [69.6%] of the total cases of NPC, while [23.4%] were negative. Positive cases included [57.2%] of keratinizing NPC, and [75%] of UNPC, meanwhile negative immunostaining for EBV-LMP1 was seen in the remaining [42.8%] of keratinizing NPC and the remaining [25%] of UNPC. The overall association of EBV-LMP1 immunoreactivity with NPC was found to be statistically significant with higher percentage of positive association among UNPC cases. Correlation of EBV-LMP1 immunoreactivity with age and .gender among cases of NPC was found to be statistically insignificant

Evaluation of tuberculosis versus pyogenic [infectious] arthritis by using MRI imaging

To assess magnetic resonance [MR] imaging features in differentiating tuberculous arthritis from pyogenic arthritis. Findings in 29 patients with tuberculous arthritis were compared with those of 13 patients with pyogenic arthritis. Bone erosion, marrow signal intensity, synovial lesion signal intensity, boundaries [smooth or irregular] for extraarticular extension of infection, and abscess rim enhancement [thin and smooth or thick and irregular] were analyzed. Revealed that bone erosion was more common in patients with tuberculous arthritis [24 [83%] of 29] than in those with pyogenic arthritis [six [46%] of 13] [P = 0.026], while subchondral marrow signal intensity abnormality was seen more frequently in patients with pyogenic arthritis [12 [92%] of 13] than in those with tuberculous arthritis [17 [59%] of 29] [P = 0.036]. On T2-weighted images, there was no significant difference between the synovial lesion signal intensities of tuberculous arthritis and pyogenic arthritis. Lesions in 16 [70%] of 23 patients with tuberculous arthritis and two [17%] of 12 patients with pyogenic arthritis had smooth extraarticular boundaries, while those in seven [30%] of 23 patients with tuberculous arthritis and 10 [83%] of 12 patients with pyogenic arthritis had irregular boundaries [P = .005]. Tuberculous abscesses [16 [100%] of 16] had thin and smooth rim enhancement, while most pyogenic abscesses [five [7 1%] of seven] had thick and irregular rims[P .001].MR imaging of bone abnormalities Tnalities, extraarticular lesions, and associated abscesses provides useful information in the differentiation of tuberculous arthritis and pyogenic arthritis

Correlative histopathological and immunohistochemical study of small in cell carcinoma, large cell carcinoma and adenocarcinoma of the lung biopsy and cytology specimens

Thirty cases of lung carcinoma specimens, collected by open biopsy [9 cases], bronchoscopic biopsy [21 cases], in additional to five cases of broncheal brush cytology and 15 cases of plural fluid aspiration cytology were examined histopathologically, cytologically and immunohistochemically. Differentiation between small and large cell carcinoma by light microscopic examination was more or less difficult especially with small biopsy specimens. Meanwhile, small cells with large amount of cytoplasm were distinguished from large cells by the assessment of the nucleus, cytoplasm, nuclear cytoplasmic ratio, cell arrangmement and the positivity of large cells to cytokeratin. Criteria for diagnosing adenocarcinoma were cell arrangement and mucin positivity by mucicarmin stain. Occasional cases show cytokeratin positive granules