ABSTRACT
The relationship between antioxidant enzyme levels and their corresponding trace elements have been extensively studied in the sera of the patients with different types of malignant tumors while few studies were reported for that relationship in the tissues of malignant tumors. To the study of the interrelationship between tissue antioxidant enzymes levels and their corresponding trace elements in different types of breast tumors. The intracellular levels of catalase, super oxide dismutase, the intracellular levels of Cu, Zn, Mn, Se and the intracellular level of malondialdehyde were determined in 29 tissue samples of patients inflicted with infiltrative ductal carcinoma and fibro adenoma. A highly significant differences were noticed in the level of the up-mentioned components in the cancerous tissues in comparison with the benign ones of the corresponding cases. The results confirmed the biochemical foundation of the oxidation theory of carcinogenesis and tumor progression On the other hand, the increase in the intracellular enzyme levels and a concomitant increase in trace elements levels can be used as a diagnostic tool in breast cancer progression and transition to malignancy
ABSTRACT
Many spectrophotometric methods were used to determine zinc in biological fluids using different reagents. Most of these methods lack specificity and sensitivity. Introduction of a new method using an azo dye called[o-carboxyphenyldiazoaminobenzene] for the determination of zinc in human serum. Different concentrations of zinc sulphate solutions were prepared. The optimum conditions for the reactions of freshly-prepared azo dye with the corresponding zinc solutions were determined. Cations interference was extensively studied. The effects of many biologically important compounds were elucidated .The results were compared with those obtained from atomic absorption spectroscopy methods using linear regression analysis. The complex formed between Zn and the corresponding dye exhibits an absorption maximum at 530 nm. Beer's law is valid over a concentration range of atomic absorption spectroscopy [0.9-8.2] micro molar. The results obtained by this method were in correlation with those obtained from. [y=0.9637x+0.0576].The interference of many cations and some biologically important compounds, if any, were hardly remarkable.This method can be anticipated with strong recommendation as an alternative to other methods which need highly sophisticated instruments due to its high accuracy, high sensitivity, specificity and selectivity with least interference compared to the other colorimetric methods