Early lipid alterations in spontaneously diabetic rats

Human and experimental diabetes mellitus extensively alters lipid metabolism. The eSS is a rat strain that develops a spontaneous diabetes of slow evolution, resembling the non-insulin-dependent diabetes mellitus of young people. We report here disturbances in lipid metabolism of 5-month old eSS rats compared to age-matched alpha-controls. Normal plasmatic glucose levels were found in the fasted state, whereas a diabetic curve was evident for eSS rats after glucose load. Triglyceride content was elevated in plasma and in liver microsomal preparations of eSS animals, when compared to the controls. The diabetic strain revealed a significant fall in the amount of linoleic acid in liver and kidney microsomes and in erythrocyte membranes. In liver, an increase in 22:6 (n-3) was also noted. A depression in the content of linoleic acid as well as an enhancement of docosahexaenoic acid were detected in phosphatidylcholine and phosphatidylethanolamine fractions from liver microsomes of eSS rats. The fatty acid pattern of eSS rat testis showed a raise in the relative percentage of arachidonic and a decrease in 22:5 (n-6), 22:5 (n-3) and 22:6 (n-3) acids compared to their controls. Diabetic rats exhibited a significant increase in microsomal cholesterol content and cholesterol/phospholipid ratio in liver and testis. In the latter tissue, higher values of fluorescence anisotropy were also observed. The current observations indicate that in early stages of the diabetes onset, when eSS rats are still normoglycemic, severe alterations of lipid metabolism may contribute to the establishment and progression of the diabetic syndrome.

Valoracion del miedo y el stress preoperatorios. Su modificacion por la conducta del anestesiologo. / Valorizacion of fear and preoperatur sress. Its modification by the behavior of the anesthesiologist

La escola valorativa del miedo (EVM) es un indicador eficaz del miedo, del paciente en el periodo prequirurgico, da evaluacion del grupo de cirugia mutilante demostro que los mismos tenian mas miedo que el grupo correspondiente a cirugia reparadora. La visita preanestesica, a traves de reducir la novedad de los estimulos atemorizantes, disminuyo el miedo de los pacientes sometidos a cirugia mutilante. Se comprobo una correlacion positiva entre los valores de la EVM y del producto de la frecuencia cardiaca por la presion arterial sistolica (FCxPA), considerado como indicador del stress. Podria concluirse que durante el periodo preoperatorio el stress aumenta en la medida en que lo hace el miedo y depende basicamente de el. La visita del anestesiologo y el uso del placebo determinaron un menor incremento de la FCxPA