Clinic-based Assessment of Folate Levels for Women of Reproductive Age, as an Impact Indicator for a National Food Fortification Program in Tanzania.

Objectives: To determine prevalence of folate deficiency and effectiveness of the National Food Fortification Program on folate levels among women of reproductive age in Tanzania. Methods: A Prospective cohort study comparing participants’ folate levels prior and gradually upon the roll-out and scale-up of the national food fortification program. A total of 636 women of reproductive age (18-49 years) enrolled and undergoing follow-up across 10 primary health facilities in Dar Es Salaam. Blood samples, food frequency, and fortified food consumption data collected at baseline (prior the program roll out), 6, 9 and 12 months. Serum folate assays performed using e411Cobas machine and results merged with food frequency and intake data. Results: We present baseline and follow-up data, estimating pre & fortification era folate levels for women of reproductive age in Tanzania. More than 25% of women have pre-fortification intake <80% their RDA for folate (mean 446, median 416, IQR ranging between 313-551micrograms). Wheat consumption is high with median portion intake of wheat-carbohydrates approximating 1 portion/day. demographics, complementing serum folate assays results will be presented. Conclusions: Low folate intake and high wheat consumption suggest potential for mandatory folate fortification of wheat in Tanzania.

Helicobacter pylori is not a Cause of Anaemia amongst People Living with HIV and AIDS in Tanzania.

Objectives: Helicobacter pylori is the most common infection worldwide. Several reports have indicated an association between H. pylori infection and anaemia. The aim of the study was to determine the prevalence of H. pylori co-infection with HIV and its correlation with anaemia and CD4 cell counts. Methods: A cross sectional study of 77 people infected with human immunodeficiency virus at SHIDEPHA+ Post Test Club Mwananyamala in Dar-es-Salaam, were assessed for Helicobacter pylori infection using 13C urea breath tests, immune integrity by use of CD4 cell count and anaemia using Beckman Coulter Counter. Results: Of the 77 subjects who were HIV positive 31 (40%) were H. pylori positive, compared to 14 out of 25 (56%) H. pylori positive amongst HIV negative controls. The mean (SD) haemoglobin level in H. pylori negative subjects was 11.7 (1.6) g/dl, compared to 11.4 (1.75) g/dl amongst those who were H. pylori positive (unpaired t test, p=0.43). Of the 15 subjects with CD4 counts below 200/ml, 4 (26%) were H. pylori positive, compared to 27/62 (48%) of those with CD4 counts above 200/ml (Chi squared, p<0.005). Conclusion: Our data demonstrates that H. pylori colonization is unlikely to contribute to anaemia amongst HIV positive subjects studied. Although this suggests that H. pylori colonization does not lead to significant problems in this population, both adequate nutrition and treatment of problems such as iron deficiency remain important components of the management of HIV positivity and AIDS throughout Africa.

Feeding practices and factors contributing to wasting, stunting, and iron-deficiency anaemia among 3-23-month old children in Kilosa district, rural Tanzania.

Infants in Tanzania are particularly vulnerable to under-nutrition during transition from breastmilk (as the only source of nourishment) to solid foods. A cross-sectional study was undertaken in Kilosa district in Tanzania to determine the feeding practices and the extent of wasting, stunting, and iron-deficiency anaemia. The study was done in two stages: in the first stage, a 24-hour dietary assessment was conducted to identify the type of complementary foods given and the eating habits according to age for 378 children aged 3-23 months. In the second stage, a progressive recruitment of 309 infants aged six months was made to measure weight, length, haemoglobin (Hb) concentration, zinc protoporphyrin concentration, and malaria parasitaemia. Birth-weight, the potential contributing factor to under-nutrition and iron-deficiency anaemia, was obtained from the children's clinic cards. The 24-hour dietary assessment revealed that children consumed mainly a thin porridge prepared from maize flour as complementary food. Carbohydrates contributed most energy (on average 69%), followed by fats (18.6%) and protein (on average 12.1%). The complementary food co-vered only 15%, 20%, and 27% of the recommended iron intake for children aged 6-8, 9-11 and 12-23 months respectively. The mean Hb concentration was 9.3 +/- 1.9 g/dL, 68% of the infants were moderately anaemic (7 < or =11 g/dL), and about 11% were severely anaemic with Hb below 7 g/dL, while 21% were non-anaemic Hb (> or =11 g/dL). Equally, the mean zinc protoporphyrin concentration was 10.0 +/- 6.2 microg/g Hb, and 76% of the infants were iron-deficient (>5 microg/g Hb). The prevalence of stunting was 35%, while wasting was only 1.3%. Low birth-weight and low body mass index of mothers were the strong predictors of stunting, whereas low birth-weight and iron-deficiency were the strong predictors of anaemia. The prevalence of malaria parasitaemia was high, affecting 50% of the infants. Having malaria was the only independent predictor associated with stunting, anaemia, and iron-deficiency. There is an urgent need to improve tradi-tional complementary foods in the studied community in terms of energy density, amount of fat in the diet, and bioavailability of macro and micronutrients.

Micronutrient dietary supplements--a new fourth approach

Currently the three main widely used strategies to control micronutrient deficiencies are food diversification, fortification, and consumption of medicinal supplements. In Tanzania a fourth strategy has been evaluated in school children, and is to be studied in pregnant and lactating women. The dietary supplement comes in the form of a powder used to prepare a fruit flavored drink. Children consumed for six months 25 grams per school day attended, the powder being added to 200 ml of water. The dietary supplement provides between 40 and 100 percent of the RDA of 10 micronutrients, which includes iron, vitamin A and iodine. Unlike medicinal supplements it provides the multiple vitamins and minerals in physiologic, not megadoses. In a well conducted randomized double blind placebo controlled trial, a dietary supplement in the form of a fortified powder fruit drink produced statistically significant differences not only in vitamin A and iron status, but also in the growth of young school age children.