ABSTRACT
Abstract Due to anthropic environmental changes, vector-borne diseases are emerging worldwide. Ticks are known vectors of several pathogens of concern among humans and animals. In recent decades, several examples of tick-borne emerging viral diseases have been reported (Crimean Congo hemorrhagic fever virus, Powassan virus, encephalitis virus, heartland virus, severe fever with thrombocytopenia syndrome virus). Unfortunately, few studies addressing the presence of viruses in wild ticks have been carried out in South America. With the aim of detecting flaviviruses and orthobunyaviruses in ticks, we carried out molecular detection in wild ticks collected in the state of Minas Gerais, Brazil. No Flavivirus-positive ticks were detected; however, we detected activity of Orthobunyavirus in 8 Amblyomma tick specimens. One of those individuals was positive for Bunyamwera orthobunyavirus, which represents the first report of this virus among ticks in South America. Further studies related to the ecology of zoonotic diseases are needed to increase knowledge of this topic, including attempts at viral isolation, full genome sequencing and biological characterization. In this way, we will obtain a better picture of the real risk of ticks as a vector for viral diseases for humans and animals on our continent, where no tick-borne viral disease is known to occur.
Resumo Alterações ambientais causadas pelo homem têm levado à emergência de doenças transmitidas por vetores no mundo. Carrapatos são vetores conhecidos de vários patógenos de importância médica e veterinária, tendo sido reportado nas últimas décadas um grande número de enfermidades virais emergentes transmitidas por eles (vírus da Febre Hemorrágica da Crimeia-Congo, vírus Powassan, vírus da Encefalite, vírus Heartland e vírus da Síndrome da Febre Trombocitopênica Severa). Infelizmente, poucos estudos envolvendo a pesquisa de vírus em carrapatos foram conduzidos na América do Sul até o momento, e nas últimas décadas um elevado número de enfermidades virais emergentes transmitidas por estes artrópodes foi relatado. Com o objetivo de investigar a presença de flavivírus e orthobunyavírus em carrapatos, foi conduzida uma análise molecular em espécimes coletados no estado de Minas Gerais, Brasil. Em nenhum carrapato foi detectada a presença de Flavivirus, no entanto, em 8 espécimes do gênero Amblyomma, foi detectada a presença de Orthobunyavirus, dos quais um espécime foi positivo para Bunyamwera orthobunyavirus. Novos estudos relacionados à ecologia de doenças zoonóticas, incluindo tentativas de isolamento viral, sequenciamento completo do genoma e caracterização biológica, são necessários. Desta forma, será possível ter uma base sobre os riscos da transmissão de vírus patogênicos por carrapatos em nosso continente, uma vez que até agora isso é desconhecido.
Subject(s)
Animals , Male , Female , Ticks/virology , Orthobunyavirus/genetics , Flavivirus/genetics , Phylogeny , Surveys and Questionnaires , Orthobunyavirus/isolation & purification , Orthobunyavirus/classification , Flavivirus/isolation & purification , Flavivirus/classificationABSTRACT
Saint Louis encephalitis virus caused an outbreak of febrile illness and encephalitis cases in Córdoba, Argentina, in 2005. During this outbreak, the strain CbaAr-4005 was isolated from Culex quinquefasciatus mosquitoes. We hypothesised that this epidemic variant would be more virulent in a mouse model than two other non-epidemic strains (78V-6507 and CorAn-9275) isolated under different epidemiological conditions. To test this hypothesis, we performed a biological characterisation in a murine model, including mortality, morbidity and infection percentages and lethal infection indices using the three strains. Mice were separated into age groups (7, 10 and 21-day-old mice) and analysed after infection. The strain CbaAr-4005 was the most infective and lethal of the three variants, whereas the other two strains exhibited a decreasing mortality percentage with increasing animal age. The strain CbaAr-4005 produced the highest morbidity percentages and no significant differences among age groups were observed. The epidemic strain caused signs of illness in all inoculated animals and showed narrower ranges from the onset of symptoms than the other strains. CbaAr-4005 was the most virulent for Swiss albino mice. Our results highlight the importance of performing biological characterisations of arbovirus strains likely to be responsible for emerging or reemerging human diseases.